Team:TUDelft/24 August 2009
Lab Notebook
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24 August 2009
Transformaltion of friday 21-08-2009 looked good. On each plate there were 80-90 kolonies
Daniel
Digestion of assemblies in order to do the second round of assembly. Gel 1% (left) for Calin´s PCR and 2% (right) for my digestions and Calin´s PCR
Right Gel (2%)
Well | Assembly | Expected Plasmid Size | Status |
1 | DNA Ladder | ✔ | |
2 | 4A | 824 | ? |
3 | 5A | 1040 | ✔ |
4 | 2A | 214 | ? |
5 | 3A | 183 | ? |
6 | 1A | 1076 | ? |
7 | PCR 1 | ||
8 | PCR 2 | ||
9 | PCR 3 | ||
10 | PCR 4 | ||
11 | PCR 5 | ||
12 | PCR 6 |