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Team:TUDelft/26 August 2009
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Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes. | Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes. | ||
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Revision as of 14:54, 17 October 2009
Lab Notebook
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26 August 2009
Orr
I helped Daniel making some plates, as well as made some more LB medium.
Saeed
8 colonies of the transformation !Z( 2 from each plate)were grown overnight for miniprep. The used colonies are named:
- !Za
- !Zb
- !Zc
- !Zd
- !Ze
- !Zf
- !Zg
- !Zh
Daniel
Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes.
