Team:TUDelft/26 August 2009

From 2009.igem.org

(Difference between revisions)
(Saeed)
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*!Zh
*!Zh
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===Daniel===
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Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes.
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Revision as of 17:06, 29 August 2009

Lab Notebook

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26 August 2009

Orr

I helped Daniel making some plates, as well as made some more LB medium.

Saeed

8 colonies of the transformation !Z( 2 from each plate)were grown overnight for miniprep. The used colonies are named:

  • !Za
  • !Zb
  • !Zc
  • !Zd
  • !Ze
  • !Zf
  • !Zg
  • !Zh

Daniel

Restriction with E and S of PCR results for Locks and Keys. Besides restriction with the same enzymes of pSB1A3 backbone. Ligation of locks and keys in Amp backbones. Transformation and culture in plates and tubes.