Team:TUDelft/30 July 2009

From 2009.igem.org

(Difference between revisions)
(Tim Vos)
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===Tim Vos===
===Tim Vos===
Minipreped 4 x pSB1C3, 4 x pSB4A5, 2 x rbs-GFP, and 4 x pSB1A3. One pSB4A5 tube is incorrect, it shows RFP expression. Also loaded the gel.
Minipreped 4 x pSB1C3, 4 x pSB4A5, 2 x rbs-GFP, and 4 x pSB1A3. One pSB4A5 tube is incorrect, it shows RFP expression. Also loaded the gel.
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 +
===Orr===
 +
Used the competent cell protocol to make cells with TSS competent.
 +
Started making a Java program that will allow lock sequence design based on the chosen RBS, and will design the equivalent key based on the lock sequence. The program will incorporate a database with all the working RBS segments in the iGEM registry, so that the user can choose which RBS he wants to use.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 21:06, 30 July 2009

Lab Notebook

July
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August
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30 July 2009

Tim Weenink

prepared assembly mixes for !B, !C and !D constructs. Standard Ginko assembly kit was used. DNA concentrations as below (these mixtures were added to 7.5 µl of digestion mixture):

Assembly name position Components volume in µl
 !B Upstream  !A DNA 33.0
H20 9.5
Downstream BBa_B0015 DNA 10.0
H20 32.5
Backbone pSB1AC3 DNA 6.5
H20 36
 !C Upstream BBa_R0040 DNA 10.0
H20 32.5
Downstream *I6 DNA 5.5
H20 37.0
Backbone pSB1AC3 DNA 6.5
H20 36
 !D Upstream *I6 DNA 5.5
H20 37.0
Downstream BBa_K145201 DNA 18.0
H20 24.5
Backbone pSB1AC3 DNA 6.5
H20 36

Ligated and transformed assemblies according to protocol.

15:20 Done plating the assemblies.

15:40 inoculated *S and *T cultures, four colonies each

16:55 Inoculated !A cultures from glycerole stock. Two tubes.

Calin

Plated three backbones left over from the transformation the other day. Did a digest on oriT-R with E and P.


Tim Vos

Minipreped 4 x pSB1C3, 4 x pSB4A5, 2 x rbs-GFP, and 4 x pSB1A3. One pSB4A5 tube is incorrect, it shows RFP expression. Also loaded the gel.

Orr

Used the competent cell protocol to make cells with TSS competent. Started making a Java program that will allow lock sequence design based on the chosen RBS, and will design the equivalent key based on the lock sequence. The program will incorporate a database with all the working RBS segments in the iGEM registry, so that the user can choose which RBS he wants to use.