Team:TUDelft/31 August 2009

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Lab Notebook

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31 August 2009

Daniel

I have some doubts about the PCR results of the locks and keys so I decided to do a second round. However, I continued the transformation of 1-4 C assemblies.

PCR purification gel (2%):

Gel310809.jpg

Well Part Expected Size Status
1
2 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
3 Lock Medium RBS 90  ?
4 Key Medium RBS 127  ?
5 Lock Weak RBS 92  ?
6 Key Weak RBS 129  ?
7 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]

I did transformation and culture in plates and tubes of 1C-4C, 1A, 1-3B and the biobricks J23119 and S03520. These last biobricks are a constitutive promoter and a LacI generator because Tim found that top 10 cells don't produce LacI