Team:TUDelft/6 August 2009

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='''6 August 2009'''=
='''6 August 2009'''=
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===Calin===
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Good results on the conjugation test. Many colonies.
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{| border="1" align="center"
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|-
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| <b>Set 1</b>
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|- align="center"
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| Plate ID || Antibiotics || Dilution || # Colonies
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|- align="center"
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| D1 || TRI || 10<sup>0</sup> || overgrown
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|- align="center"
 +
| D2 || TRI || 10<sup>-2</sup>|| 6770
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|- align="center"
 +
| D3 || TRI || 10<sup>-4</sup>|| 170
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|- align="center"
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| D4 || TRI || 10<sup>-5</sup>|| 40
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|- align="center"
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| D5 || TRI || 10<sup>-6</sup>|| 10
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|- align="center"
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| T1 || TRI + AMP || 10<sup>0</sup> || ?
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|- align="center"
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| T2 || TRI + AMP || 10<sup>-1</sup>|| ?
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|- align="center"
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| T3 || TRI + AMP || 10<sup>-2</sup>|| 186
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|- align="center"
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| T4 || TRI + AMP || 10<sup>-3</sup>|| 11
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|- align="center"
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| T5 || TRI + AMP || 10<sup>-4</sup>|| 0
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|- align="center"
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| T6 || TRI + AMP || 10<sup>-5</sup> || 0
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|- align="center"
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| T7 || TRI + AMP || 10<sup>-6</sup>|| 0
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|- align="center"
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| R1 || AMP || 10<sup>0</sup> || overgrown
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|- align="center"
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| R2 || AMP || 10<sup>-4</sup>|| 32
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|- align="center"
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| R3 || AMP || 10<sup>-5</sup>|| 6
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|- align="center"
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| R4 || AMP || 10<sup>-6</sup>|| 2
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|}
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<br><br>
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{| border="1" align="center"
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|-
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| <b>Set 2</b>
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|- align="center"
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| Plate ID || Antibiotics || Dilution ||
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|- align="center"
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| D6 || TRI || 10<sup>0</sup> || overgrown
 +
|- align="center"
 +
| D7 || TRI || 10<sup>-2</sup>|| 8320
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|- align="center"
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| D8 || TRI || 10<sup>-4</sup>|| 114
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|- align="center"
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| D9 || TRI || 10<sup>-5</sup>|| 18
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|- align="center"
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| D10 || TRI || 10<sup>-6</sup>|| 8
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|- align="center"
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| T8 || TRI + AMP || 10<sup>0</sup> || ?
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|- align="center"
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| T9 || TRI + AMP || 10<sup>-1</sup>|| ?
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|- align="center"
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| T10 || TRI + AMP || 10<sup>-2</sup>|| 228
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|- align="center"
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| T11 || TRI + AMP || 10<sup>-3</sup>|| 18
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|- align="center"
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| T12 || TRI + AMP || 10<sup>-4</sup>|| 2
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|- align="center"
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| T13 || TRI + AMP || 10<sup>-5</sup> || 0
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|- align="center"
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| T14 || TRI + AMP || 10<sup>-6</sup>|| 0
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|}
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<br><br>
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{| border="1" align="center"
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|-
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| <b>Set 3</b>
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|- align="center"
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| Plate ID || Antibiotics || Dilution ||
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|- align="center"
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| D11 || TRI || 10<sup>0</sup> || overgrown
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|- align="center"
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| D12 || TRI || 10<sup>-2</sup>|| 8950
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|- align="center"
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| D13 || TRI || 10<sup>-4</sup>|| 173
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|- align="center"
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| D14 || TRI || 10<sup>-5</sup>|| 22
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|- align="center"
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| D15 || TRI || 10<sup>-6</sup>|| 1
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|- align="center"
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| T15 || TRI + AMP || 10<sup>0</sup> || ?
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|- align="center"
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| T16 || TRI + AMP || 10<sup>-1</sup>|| ?
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|- align="center"
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| T17 || TRI + AMP || 10<sup>-2</sup>|| 218
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|- align="center"
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| T18 || TRI + AMP || 10<sup>-3</sup>|| 31
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|- align="center"
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| T19 || TRI + AMP || 10<sup>-4</sup>|| 2
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|- align="center"
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| T20 || TRI + AMP || 10<sup>-5</sup> || 0
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|- align="center"
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| T21 || TRI + AMP || 10<sup>-6</sup>|| 0
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|}
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<br><br>
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Calculated conjugation efficiencies:
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{| border="1" align="center"
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|- align="center"
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| Set || Conjugation Efficiency
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|- align="center"
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| 1 ||  0.028
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|- align="center"
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| 2 ||  0.028
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|- align="center"
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| 3 ||  0.025
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|}
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Trimethoprim control plate had no growth.
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5mL tube of assembly CA made.
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Elecroporated 10 assemblies.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 19:04, 6 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

6 August 2009

Calin

Good results on the conjugation test. Many colonies.


Set 1
Plate ID Antibiotics Dilution # Colonies
D1 TRI 100 overgrown
D2 TRI 10-2 6770
D3 TRI 10-4 170
D4 TRI 10-5 40
D5 TRI 10-6 10
T1 TRI + AMP 100  ?
T2 TRI + AMP 10-1 ?
T3 TRI + AMP 10-2 186
T4 TRI + AMP 10-3 11
T5 TRI + AMP 10-4 0
T6 TRI + AMP 10-5 0
T7 TRI + AMP 10-6 0
R1 AMP 100 overgrown
R2 AMP 10-4 32
R3 AMP 10-5 6
R4 AMP 10-6 2



Set 2
Plate ID Antibiotics Dilution
D6 TRI 100 overgrown
D7 TRI 10-2 8320
D8 TRI 10-4 114
D9 TRI 10-5 18
D10 TRI 10-6 8
T8 TRI + AMP 100  ?
T9 TRI + AMP 10-1 ?
T10 TRI + AMP 10-2 228
T11 TRI + AMP 10-3 18
T12 TRI + AMP 10-4 2
T13 TRI + AMP 10-5 0
T14 TRI + AMP 10-6 0



Set 3
Plate ID Antibiotics Dilution
D11 TRI 100 overgrown
D12 TRI 10-2 8950
D13 TRI 10-4 173
D14 TRI 10-5 22
D15 TRI 10-6 1
T15 TRI + AMP 100  ?
T16 TRI + AMP 10-1 ?
T17 TRI + AMP 10-2 218
T18 TRI + AMP 10-3 31
T19 TRI + AMP 10-4 2
T20 TRI + AMP 10-5 0
T21 TRI + AMP 10-6 0



Calculated conjugation efficiencies:

Set Conjugation Efficiency
1 0.028
2 0.028
3 0.025

Trimethoprim control plate had no growth.

5mL tube of assembly CA made.

Elecroporated 10 assemblies.

Retrieved from "http://2009.igem.org/Team:TUDelft/6_August_2009"