Team:TUDelft/6 August 2009

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Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

6 August 2009

Calin

Good results on the conjugation test. Many colonies.


Set 1
Plate ID Antibiotics Dilution # Colonies
D1 TRI 100 overgrown
D2 TRI 10-2 6770
D3 TRI 10-4 170
D4 TRI 10-5 40
D5 TRI 10-6 10
T1 TRI + AMP 100  ?
T2 TRI + AMP 10-1 ?
T3 TRI + AMP 10-2 186
T4 TRI + AMP 10-3 11
T5 TRI + AMP 10-4 0
T6 TRI + AMP 10-5 0
T7 TRI + AMP 10-6 0
R1 AMP 100 overgrown
R2 AMP 10-4 32
R3 AMP 10-5 6
R4 AMP 10-6 2



Set 2
Plate ID Antibiotics Dilution
D6 TRI 100 overgrown
D7 TRI 10-2 8320
D8 TRI 10-4 114
D9 TRI 10-5 18
D10 TRI 10-6 8
T8 TRI + AMP 100  ?
T9 TRI + AMP 10-1 ?
T10 TRI + AMP 10-2 228
T11 TRI + AMP 10-3 18
T12 TRI + AMP 10-4 2
T13 TRI + AMP 10-5 0
T14 TRI + AMP 10-6 0



Set 3
Plate ID Antibiotics Dilution
D11 TRI 100 overgrown
D12 TRI 10-2 8950
D13 TRI 10-4 173
D14 TRI 10-5 22
D15 TRI 10-6 1
T15 TRI + AMP 100  ?
T16 TRI + AMP 10-1 ?
T17 TRI + AMP 10-2 218
T18 TRI + AMP 10-3 31
T19 TRI + AMP 10-4 2
T20 TRI + AMP 10-5 0
T21 TRI + AMP 10-6 0



Calculated conjugation efficiencies:

Set Conjugation Efficiency
1 0.028
2 0.028
3 0.025

Trimethoprim control plate had no growth.

5mL tube of assembly CA made.

Elecroporated 10 assemblies.

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