Team:TUDelft/7 September 2009

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Lab Notebook

July
MTWTFSS
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6 7 8 9 10 11 12
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20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

7 September 2009

Saeed

upstream restircted !P1 was used for assembly with downstream double terminator. After the assembly the plasmide was electro transformed. Cell are plated on medium containing CAM.

Today I also count the colonies of Tim Weeniks tranformation of Friday 04-09-2009.

Transformation plate number number of colonies
 !SIII A1 1 1
 !SIII A1 2 27
 !SIII A2 1 24
 !SIII A2 2 6
 !SIII A3 1 50
 !SIII A3 2 2
 !SIII A4 1 47
 !SIII A4 2 150+

I made 10 overnight cultures form these plates, I picked one colonie from each plate. From both A4 plates I picked two colonies.