Lab Notebook
7 September 2009
Saeed
upstream restircted !P1 was used for assembly with downstream double terminator. After the assembly the plasmide was electro transformed. Cell are plated on medium containing CAM.
Today I also count the colonies of Tim Weeninks tranformation of Friday 04-09-2009.
| Transformation | plate number | number of colonies
|
| !SIII A1 | 1 | 1
|
| !SIII A1 | 2 | 27
|
| !SIII A2 | 1 | 24
|
| !SIII A2 | 2 | 6
|
| !SIII A3 | 1 | 50
|
| !SIII A3 | 2 | 2
|
| !SIII A4 | 1 | 47
|
| !SIII A4 | 2 | 150+
|
I made 10 overnight cultures form these plates, I picked one colonie from each plate. From both A4 plates I picked two colonies.
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