Team:UNICAMP-Brazil/Protocols

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(Difference between revisions)

Latest revision as of 18:55, 21 October 2009

Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution

13. Preparation of electrocompetent S. cerevisiae

14. Restriction Reaction

15. pGEM cloning strategy

16. Expression strategy in Yeast

@@ Line 8: / Line 8: @@
 '''[[Team:UNICAMP-Brazil/Protocols/Mini-Prep|2. Mini-Prep]]'''
-'''[[Team:UNICAMP-Brazil/Protocols/Electroporation|3. Electroporation]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/Electroporation|3. Transformation]]'''
 '''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent E. coli|4. Preparation of electrocompetent ''E. coli'']]'''
@@ Line 15: / Line 15: @@
 '''[[Team:UNICAMP-Brazil/Protocols/Yeast RNA Extraction|6. Yeast RNA Extraction]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/Purification of DNA fragments from agarose gels|7. Purification of DNA fragments from agarose gels]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/RNA isolation from 'Gallus gallus' spleen, blood and oviduct|8. Isolation of RNA using Trizol Reagent (Invitrogen)]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/SAP Dephosphorylation|9. SAP Dephosphorylation]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/CIAP Dephosphorylation|10. CIAP Dephosphorylation]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/T4 DNA Ligase|11. T4 DNA Ligase]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/Electroelution|12. Electroelution]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent ''S. cerevisiae'']]'''
+'''[[Team:UNICAMP-Brazil/Protocols/Restriction reaction|14. Restriction Reaction]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/pGEMStrategy|15. pGEM cloning strategy]]'''
+'''[[Team:UNICAMP-Brazil/Protocols/YEP|16. Expression strategy in Yeast]]'''
 {{:Team:UNICAMP-Brazil/inc_rodape}}