Team:UQ-Australia/Notebook
From 2009.igem.org
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Mini prep DNA extractions performed for MS427-pBAD, MS427-pKKJ143 cultures incubated overnight.<br/> | Mini prep DNA extractions performed for MS427-pBAD, MS427-pKKJ143 cultures incubated overnight.<br/> | ||
--> run on ethidium bromide gel to confirm DNA production.<br/> | --> run on ethidium bromide gel to confirm DNA production.<br/> | ||
- | Click[https://2009.igem.org/Image:UQ_MercuryMiniprep_AgaroseGel_1.png HERE] for a picture of the gel. | + | Click [https://2009.igem.org/Image:UQ_MercuryMiniprep_AgaroseGel_1.png HERE] for a picture of the gel. |
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Revision as of 00:21, 19 August 2009
Water Purification Project
24/07/09 - DNA extraction and electrophoresis 23/07/09 - Transformations with AG43 plasmids Strain: MS427
Strain: MS427 17/07/09 - MG1655 Stock Preparation
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Bioprecipitation Project
11/08/09 No results were given from the transformation. Transformation will have to be repeated.
To see protocol, click HERE
LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.
Plasmids were stored and transformation will be done on Monday.
Plasmids have arrived! We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution. A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight. Click on the plasmid name to look at the vector |