Team:UQ-Australia/Notebook
From 2009.igem.org
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'''13/08/09''' | '''13/08/09''' | ||
- | A second transformation was performed on the four plasmids. | + | A second transformation was performed on the four plasmids. Procedure slightly changed, click [https://2009.igem.org/Team:UQ-Australia/Notebook/Transformation_procedure2 HERE] to see it. |
'''11/08/09''' | '''11/08/09''' |
Revision as of 05:27, 2 September 2009
Water Purification Project
01/09/09 - Agarose Gel and Nanodrop 28/08/09 - DNA miniprep EDIT (01/09/09): these tubes now in IGEM Green Box ("Fiona" freezer, bottom shelf).
27/08/09 - stuff 26/08/09 - stuff 25/08/09 - stuff 24/07/09 - DNA extraction and electrophoresis 23/07/09 - Transformations with AG43 plasmids Strain: MS427
Strain: MS427 17/07/09 - MG1655 Stock Preparation
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Bioprecipitation Project13/08/09 A second transformation was performed on the four plasmids. Procedure slightly changed, click HERE to see it. 11/08/09 No results were given from the transformation. Transformation will have to be repeated.
To see protocol, click HERE
LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.
Plasmids were stored and transformation will be done on Monday.
Plasmids have arrived! We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution. A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight. Click on the plasmid name to look at the vector |