User:DavidC/18 October 2009
From 2009.igem.org
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+ | === Tuesday the 13th to Sunday the 18th === | ||
+ | |||
+ | Growth of bacteria in the goal to extract the bio-bricks: <br> | ||
+ | |||
+ | BBa_J23100 + BBa_B0014; <br> | ||
+ | BBa_B0030 + BBa_J23100 + BBa_B0014; <br> | ||
+ | BBa_R0010 + BBa_B0030; <br> | ||
+ | BBa_P1003 + BBa_B0014; <br> | ||
+ | BBa_B0014 + BBa_P1003 + BBa_B0014; <br> | ||
+ | BBa_C0012 + BBa_B0014; <br> | ||
+ | BBa_B0030 + BBa_C0012 + BBa_B0014; <br> | ||
+ | BBa_I712669 + BBa_B0014; <br> | ||
+ | |||
+ | 5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL). <br> | ||
+ | |||
+ | ==== DNA extraction ==== | ||
+ | |||
+ | By using miniprep from promega: | ||
+ | |||
+ | BBa_J23100 + BBa_B0014; <br> | ||
+ | BBa_B0030 + BBa_J23100 + BBa_B0014; <br> | ||
+ | BBa_R0010 + BBa_B0030; <br> | ||
+ | BBa_P1003 + BBa_B0014; <br> | ||
+ | BBa_B0014 + BBa_P1003 + BBa_B0014; <br> | ||
+ | BBa_C0012 + BBa_B0014; <br> | ||
+ | BBa_B0030 + BBa_C0012 + BBa_B0014; <br> | ||
+ | BBa_I712669 + BBa_B0014; <br> | ||
+ | D protein from lambda phage | ||
+ | |||
+ | ==== Quantification ==== | ||
+ | |||
+ | Quantification of miniprep with an electrophoresis. <br> | ||
+ | Mix : 2µL of loading buffer + 2 µL of DNA. <br> | ||
+ | |||
+ | 85 Volt, 15 minutes. <br> | ||
+ | 105 Volt, 40 minutes. <br> | ||
+ | Ladder fermentas 1 Kb. <br> |
Latest revision as of 04:41, 21 October 2009
Tuesday the 13th to Sunday the 18th
Growth of bacteria in the goal to extract the bio-bricks:
BBa_J23100 + BBa_B0014;
BBa_B0030 + BBa_J23100 + BBa_B0014;
BBa_R0010 + BBa_B0030;
BBa_P1003 + BBa_B0014;
BBa_B0014 + BBa_P1003 + BBa_B0014;
BBa_C0012 + BBa_B0014;
BBa_B0030 + BBa_C0012 + BBa_B0014;
BBa_I712669 + BBa_B0014;
5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL).
DNA extraction
By using miniprep from promega:
BBa_J23100 + BBa_B0014;
BBa_B0030 + BBa_J23100 + BBa_B0014;
BBa_R0010 + BBa_B0030;
BBa_P1003 + BBa_B0014;
BBa_B0014 + BBa_P1003 + BBa_B0014;
BBa_C0012 + BBa_B0014;
BBa_B0030 + BBa_C0012 + BBa_B0014;
BBa_I712669 + BBa_B0014;
D protein from lambda phage
Quantification
Quantification of miniprep with an electrophoresis.
Mix : 2µL of loading buffer + 2 µL of DNA.
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.