User:DavidC/19 August 2009
From 2009.igem.org
(Difference between revisions)
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<i>Mycobacterium avium</i> transplanting on Lowensteim medium (two tubes). <br> | <i>Mycobacterium avium</i> transplanting on Lowensteim medium (two tubes). <br> | ||
- | ==== First polymerase chain reaction (PCR) on the lambda phage D protein. ==== | + | ==== First polymerase chain reaction (PCR) on the lambda phage D protein. ==== |
Cycle: <br> | Cycle: <br> | ||
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Primers extension : 72°C, 1 minute. <br> | Primers extension : 72°C, 1 minute. <br> | ||
- | ==== Electrophoresis | + | ==== Electrophoresis ==== |
- | + | ||
- | + | ||
85 Volt, 15 minutes. <br> | 85 Volt, 15 minutes. <br> |
Latest revision as of 01:28, 21 October 2009
Wednesday the 19th
Mycobacterium avium transplanting on Lowensteim medium (two tubes).
First polymerase chain reaction (PCR) on the lambda phage D protein.
Cycle:
Hot-Start : 94°C, 2 minutes.
DNA denaturation : 94°C, 1 minute.
Primers annealing : 59°C, 1 minute.
Primers extension : 72°C, 1 minute.
Electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Sample : D protein
No results.