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User:DavidC/21 August 2009
From 2009.igem.org
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| + | === Friday the 21th === | ||
| + | |||
| + | ==== PCR ==== | ||
| + | |||
| + | PCR of adenovirus 5 penton base from plasmid coding for the virus.<br><br> | ||
| + | |||
| + | 94°C, 2 minute. <br> | ||
| + | (94°C, 1 minute. <br> | ||
| + | 55°C, 1 minutes. <br> | ||
| + | 72°C, 3 minutes.) X 5 <br> | ||
| + | 72°C, 5 minutes. <br> | ||
| + | (94°C, 1 minute. <br> | ||
| + | 66°C, 1 minutes. <br> | ||
| + | 72°C, 3 minutes.) X 30 <br><br> | ||
| + | |||
| + | ==== DNA electrophoresis ==== | ||
| + | 85 Volt, 15 minutes. <br> | ||
| + | 105 Volt, 40 minutes. <br> | ||
| + | Ladder fermentas 1 Kb. <br> | ||
| + | |||
| + | No amplification of the adenovirus 5 penton base, we have mismatch on the second trial. <br> | ||
Revision as of 01:32, 21 October 2009
Project
Introduction Current therapies Gene therapies DVS Concept Tissue Vector Cell Vector Therapeutic plasmid Conclusion Implementation Tissue targeting Antitumor action Treatment modeling Conclusion
Ressources
BioBricks Material & Method Bibliography
Ethics & Safety
Friday the 21th
PCR
PCR of adenovirus 5 penton base from plasmid coding for the virus.
94°C, 2 minute.
(94°C, 1 minute.
55°C, 1 minutes.
72°C, 3 minutes.) X 5
72°C, 5 minutes.
(94°C, 1 minute.
66°C, 1 minutes.
72°C, 3 minutes.) X 30
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
No amplification of the adenovirus 5 penton base, we have mismatch on the second trial.
