User:DavidC/21 August 2009
From 2009.igem.org
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No amplification of the adenovirus 5 penton base, we have mismatch on the second trial. <br> | No amplification of the adenovirus 5 penton base, we have mismatch on the second trial. <br> | ||
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+ | ==== Lambda phage extraction ==== | ||
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+ | Lambda phage extraction. 2 dishes, at 12 and 24 hours of incubation, dilution = 1/10 and 1/10 000. <br> | ||
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+ | Growth of lambda phages for extraction. 5 dishes with <i>E.coli</i> infected by Lambda phages, dilution = 1/10. <br> |
Revision as of 01:33, 21 October 2009
Contents |
Friday the 21th
PCR
PCR of adenovirus 5 penton base from plasmid coding for the virus.
94°C, 2 minute.
(94°C, 1 minute.
55°C, 1 minutes.
72°C, 3 minutes.) X 5
72°C, 5 minutes.
(94°C, 1 minute.
66°C, 1 minutes.
72°C, 3 minutes.) X 30
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
sample: adenovirus penton base (ADV5pb)
No amplification of the adenovirus 5 penton base, we have mismatch on the second trial.
Lambda phage extraction
Lambda phage extraction. 2 dishes, at 12 and 24 hours of incubation, dilution = 1/10 and 1/10 000.
Growth of lambda phages for extraction. 5 dishes with E.coli infected by Lambda phages, dilution = 1/10.