User:DavidC/23 August 2009
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Lambda phage extraction. <br> | Lambda phage extraction. <br> | ||
+ | |||
+ | === PCR ==== | ||
+ | |||
+ | Sample: D protein. <br> | ||
+ | |||
+ | Cycle : <br> | ||
+ | |||
+ | 94°C 2minutes; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 59°C 1 minutes; <br> | ||
+ | 72°C 1 minute(s)) X 35; <br> | ||
+ | 72°C 4 minutes. <br> | ||
+ | |||
+ | Samples: Adenovirus 5, penton base. <br> | ||
+ | |||
+ | Cycle : <br> | ||
+ | |||
+ | 94°C 2minutes; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 62°C 1 minutes; <br> | ||
+ | 72°C 3 minute(s)) X 35; <br> | ||
+ | 72°C 6 minutes. <br> | ||
+ | |||
+ | |||
+ | Samples: COS sequence from Lambda phage. <br> | ||
+ | |||
+ | Cycle : <br> | ||
+ | |||
+ | 94°C 2minutes; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 57°C 1 minutes; <br> | ||
+ | 72°C 1 minute(s)) X 35; <br> | ||
+ | 72°C 4 minutes. <br> | ||
+ | |||
+ | |||
+ | Samples: CI repressor. <br> | ||
+ | |||
+ | Cycle : <br> | ||
+ | |||
+ | 94°C 2minutes; <br> | ||
+ | (94°C 1 minute; <br> | ||
+ | 57°C 1 minutes; <br> | ||
+ | 72°C 3 minute(s)) X 35; <br> | ||
+ | 72°C 6 minutes. <br> | ||
+ | |||
+ | ==== DNA electrophoresis ==== | ||
+ | |||
+ | 85 Volt, 15 minutes. <br> | ||
+ | 105 Volt, 40 minutes. <br> | ||
+ | Ladder fermentas 1 Kb. <br> | ||
+ | |||
+ | Samples: D protein (D prt)385bp, adenovirus penton base (ADV5pb) 1725bp, COS sequence 250bp and CI repressor 713bp. <br> | ||
+ | |||
+ | [[image:SU2308.png|center]] <br> | ||
+ | |||
+ | [[image:SU2308(2).png|center]] <br> |
Latest revision as of 14:17, 21 October 2009
Sunday the 23th
Lambda phage extraction.
PCR =
Sample: D protein.
Cycle :
94°C 2minutes;
(94°C 1 minute;
59°C 1 minutes;
72°C 1 minute(s)) X 35;
72°C 4 minutes.
Samples: Adenovirus 5, penton base.
Cycle :
94°C 2minutes;
(94°C 1 minute;
62°C 1 minutes;
72°C 3 minute(s)) X 35;
72°C 6 minutes.
Samples: COS sequence from Lambda phage.
Cycle :
94°C 2minutes;
(94°C 1 minute;
57°C 1 minutes;
72°C 1 minute(s)) X 35;
72°C 4 minutes.
Samples: CI repressor.
Cycle :
94°C 2minutes;
(94°C 1 minute;
57°C 1 minutes;
72°C 3 minute(s)) X 35;
72°C 6 minutes.
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples: D protein (D prt)385bp, adenovirus penton base (ADV5pb) 1725bp, COS sequence 250bp and CI repressor 713bp.