User:DavidC/23 August 2009
From 2009.igem.org
Sunday the 23th
Lambda phage extraction.
PCR =
Sample: D protein.
Cycle :
94°C 2minutes;
(94°C 1 minute;
59°C 1 minutes;
72°C 1 minute(s)) X 35;
72°C 4 minutes.
Samples: Adenovirus 5, penton base.
Cycle :
94°C 2minutes;
(94°C 1 minute;
62°C 1 minutes;
72°C 3 minute(s)) X 35;
72°C 6 minutes.
Samples: COS sequence from Lambda phage.
Cycle :
94°C 2minutes;
(94°C 1 minute;
57°C 1 minutes;
72°C 1 minute(s)) X 35;
72°C 4 minutes.
Samples: CI repressor.
Cycle :
94°C 2minutes;
(94°C 1 minute;
57°C 1 minutes;
72°C 3 minute(s)) X 35;
72°C 6 minutes.
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples: D protein (D prt)385bp, adenovirus penton base (ADV5pb) 1725bp, COS sequence 250bp and CI repressor 713bp.