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User:DavidC/24 September 2009
From 2009.igem.org
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| + | === Thursday the 24th === | ||
| + | |||
| + | ==== Restriction ==== | ||
| + | |||
| + | ==== Ligation of P1003 with B0014 ==== | ||
| + | |||
| + | |||
| + | ==== Ligation of C0012 with B0014 ==== | ||
| + | |||
| + | |||
| + | ==== Ligation of B0030 with B0014 + J23100 ==== | ||
| + | |||
| + | |||
| + | ==== Ligation of R0010 with B0014 ==== | ||
| + | |||
| + | |||
| + | ==== Ligation of I712612 with B0014 ==== | ||
| + | |||
| + | |||
| + | ==== DNA electrophoresis ==== | ||
| + | |||
| + | 85 Volt, 15 minutes. <br> | ||
| + | 105 Volt, 40 minutes. <br> | ||
| + | Ladder fermentas 1 Kb. <br> | ||
| + | |||
| + | |||
| + | Samples: | ||
| + | |||
| + | ==== DNA purification ==== | ||
| + | |||
| + | Kit Qiagen “gel extraction kit”, final volume = 50µL. <br> | ||
Revision as of 03:36, 21 October 2009
Project
Introduction Current therapies Gene therapies DVS Concept Tissue Vector Cell Vector Therapeutic plasmid Conclusion Implementation Tissue targeting Antitumor action Treatment modeling Conclusion
Ressources
BioBricks Material & Method Bibliography
Ethics & Safety
Contents |
Thursday the 24th
Restriction
Ligation of P1003 with B0014
Ligation of C0012 with B0014
Ligation of B0030 with B0014 + J23100
Ligation of R0010 with B0014
Ligation of I712612 with B0014
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples:
DNA purification
Kit Qiagen “gel extraction kit”, final volume = 50µL.
