User:DavidC/24 September 2009
From 2009.igem.org
Contents |
Thursday the 24th
Restriction
Ligation of P1003 with B0014
Ligation of C0012 with B0014
Ligation of B0030 with B0014 + J23100
Ligation of R0010 with B0014
Ligation of I712612 with B0014
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
Samples:
DNA purification
Kit Qiagen “gel extraction kit”, final volume = 50µL.