User:DavidC/24 September 2009

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framless



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Contents

Thursday the 24th

Restriction digest

Ligation between BBa_P1003 and BBa_B0014

Ligation between BBa_C0012 and BBa_B0014

Ligation between BBa_B0030 and BBa_B0014 + BBa_J23100

Ligation between BBa_R0010 and BBa_B0014

Ligation between BBa_I712612 and BBa_B0014

DNA electrophoresis

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.


Samples:

DNA purification

Kit Qiagen “gel extraction kit”, final volume = 50µL.

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