Team:Groningen/Notebook/1 August 2009

From 2009.igem.org

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Wet

Miscellaneous

ON Cultures:
KH1: No growth
KL1: No growth
KL2: No growth
AL1: No growth
AH2: Minimal growth
Left the ones with no growth on the bench, de rest was spinned down and pellets frozen.
Pellets of AL2, KM1, KH2 & AH1 were red of colour.

Plates:
Glpf in pSB1AC (31/07/09, TY amp120) & Glpf con. pSB1AC (31/07/09, TY amp120) both Empty, left them on the bench.
A lot of other plates in incubator I was not told of, left them in the incubator (No growth)

GVP Cluster

Transporters

Metal Accumulation

Vectors

Transformation
+ controls are completely full.
Zn-RBS (50 μL) is covered in growth, no single colonies comparable to positive controls (200 μL).
Other plates seem to have no colonies, at least not identifiable next to agar debris on plates (bad quality plates). If these were to be identified as colonies they are also present on negative controls (MilliQ & Selfcloser).
Plates are left on the bench, Zn-RBS is put to 4 °C

Dry

April
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30