Team:KULeuven/5 August 2009

From 2009.igem.org

Project progress

Progress of parts

[edit] Blue Light Receptor

[edit] Vanillin Production

Miniprep of pairs:


Restriction digestion
A1: sam8, EcoRI en SpeI
A2: sam5, EcoRI en XbaI

B1: RBS, EcoRI en SpeI
B2: COMT, EcoRI en XbaI

C1: fcs, EcoRI en SpeI
C2: ech, EcoRI en XbaI

The mixture was incubated in 37°C for 1h30...

...followed by gel electrophoresis of the digested parts

[edit] Vanillin Receptor

  • Gel electrophoresis was done to see if PCR has worked. VirA, virB and rpoA showed a clear result. We performed miniprep on those genes to purify and clear them. VirG didn't show any result.
  • We did a new PCR for all the genes and especially for virG with a better annealing temperature. The gel electrophoresis showed a good and clear result for all the genes.

[edit] Key/Lock/Anti-Key