Team:Kyoto/GSDD/Notebook/0727-0731

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0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0727-0731 : what to do. what to do. what to do. what to do.


TuesDay, 28 July - Wednesday, 29 July

To Do

  • Transformation
    • 3-M-9
    • 3-I-9
    • 1-A-4
    • 1-O-2
    • 3-O-22(retry)
    • 3-M-2(retry)
    • 3-O-17(retry)


Results

Kyoto 0728.png

  • Colony
3-M-9
None
3-I-9
Observed
1-A-4
Observed
1-O-2
Observed
3-O-22(retry)
None
3-M-2(retry)
Observed
3-O-17(retry)
None



Thursday, 30 July

To Do

  • Transformation
    • 3-O-17(retry)
    • 3-M-9(retry)


Results

Kyoto 0730.jpg

  • Colony
3-O-17(retry)
None
3-M-9(retry)
Observed



Thursday, 30 July

To Do

  • Miniprep Kit
  • Restriction Enzyme Digestion (EcoR1, EcoR1 + Spe1)
  • Electrophoresis
    • 1-a-4
    • 1-0-2
    • 3-m-2
    • 3-m-9
    • 3-i-9


Results

Miniprep conc.
conc.
NO.Sample nameconc./(ng/ul)
11-a-464.1
21-o-273.6
33-m-296.5
43-m-958.6
53-i-959.0
Electrophoresis

Kyoto 0731.png

  • No bands were observed.
conc.
NO.Sample nameparts length/bpplasmid length/bp
11-a-46602079
21-o-211282079
33-m-22532077
43-m-92282079
53-i-94382079