Team:Groningen/Notebook/18 August 2009
From 2009.igem.org
(Difference between revisions)
(→Transporters) |
(→Transporters) |
||
Line 6: | Line 6: | ||
===Transporters=== | ===Transporters=== | ||
- | HmtA curios about the dirty experiment. a 1% gel control will show if expected product is there. | + | HmtA curios about the dirty experiment. a 1% gel control will show if expected product is there. Unfortunately there was no product, the primer dimer changed and pcr 1 and 2 remain available. PCR again. Different program. |
+ | Plus starting an other PCR1 and PCR2 to get new megaprimers. | ||
===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 08:59, 18 August 2009
Wet
GVP Cluster
Transporters
HmtA curios about the dirty experiment. a 1% gel control will show if expected product is there. Unfortunately there was no product, the primer dimer changed and pcr 1 and 2 remain available. PCR again. Different program. Plus starting an other PCR1 and PCR2 to get new megaprimers.
Metal Accumulation
Restriction analysis MBP-ArsR
Vectors
Restriction analysis metal promotors
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|