Team:Groningen/Notebook/20 August 2009

From 2009.igem.org

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[[Image:pNL29 IMG_3823.JPG|200px|thumb|left|Red is negtive controle, 1mm full floating and 0,1 mm same level als controle]]
[[Image:pNL29 IMG_3823.JPG|200px|thumb|left|Red is negtive controle, 1mm full floating and 0,1 mm same level als controle]]
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===Transporters===
===Transporters===

Revision as of 12:57, 21 August 2009

Igemhomelogo.png

Meeting iGEM teams at TU delft

Presentation given in Delft.

Wet

GVP Cluster

Trying to get a nice picture of the tubes but they are not very photogenic.

Zn seems to work in a gradient accept for the tube without Zn.
increase concentration, red culture negative contole
Ars all are floating (could be very leaky/constituitive)
increase concentration, full floating. red culture negative contol
Cu shows a gradient effect
increase concentration, red culture negative contole
pNL29 shows a clear floating difference
Red is negtive controle, 1mm full floating and 0,1 mm same level als controle


Transporters

Due to the mutation. restarted cloning again with PCR on pBAD plasmid with primers F2 rev

Metal Accumulation

Vectors

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30