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Team:Groningen/Notebook/22 July 2009
From 2009.igem.org
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| - | :: Mix gently and spin down | + | ::* Mix gently and spin down |
| - | ::incubate at 37° for 30 min. | + | ::* incubate at 37° for 30 min. |
==Dry== | ==Dry== | ||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} | ||
Revision as of 12:03, 22 July 2009
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Wet
GVP Cluster
Transporters
Metal Accumulation
Vectors
In order to check the VR VF2 primers that were ordered this year a gradient PCR was done with a vector that is expected to work (pSB3K3), a pUC vector and a negative control with no vector.
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As a check-up we also did a restrition analysis for the sPB3K3 vector with EcoR1 and Spe1
| 10x fast digest buffer | 2 uL |
| pSB3K3 (22,5 ngr/uL) | 8 uL (=180ngr up to 1ugr is recommended at fermentas) |
| EcoR1 | 1 uL |
| Spe1 | 1 uL |
| MilliQ | 8 uL |
| Total | 20 uL |
- Mix gently and spin down
- incubate at 37° for 30 min.
Dry
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