Team:Groningen/Notebook/22 July 2009
From 2009.igem.org
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::* Mix gently and spin down | ::* Mix gently and spin down | ||
::* incubate at 37° for 30 min. | ::* incubate at 37° for 30 min. | ||
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+ | Results of the PCR and restiction shown below. | ||
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+ | [[image:F102471_2009-07-22_07hr_04min_ControlVRVF2_noted.jpg]] | ||
==Dry== | ==Dry== | ||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} |
Revision as of 14:41, 22 July 2009
Wet
GVP Cluster
Transporters
Metal Accumulation
Vectors
In order to check the VR VF2 primers that were ordered this year a gradient PCR was done with a vector that is expected to work (pSB3K3), a pUC vector and a negative control with no vector.
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As a check-up we also did a restrition analysis for the sPB3K3 vector with EcoR1 and Spe1
10x fast digest buffer | 2 uL |
pSB3K3 (22,5 ngr/uL) | 8 uL (=180ngr up to 1ugr is recommended at fermentas) |
EcoR1 | 1 uL |
Spe1 | 1 uL |
MilliQ | 8 uL |
Total | 20 uL |
- Mix gently and spin down
- incubate at 37° for 30 min.
Results of the PCR and restiction shown below.
Dry
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