Team:Groningen/Notebook/23 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== ===Vectors=== {| | <!--Tabel 1 hier--> |width="10"| | {| border="1" |+ ''...)
(→Vectors)
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| 21 uL
| 21 uL
|-
|-
-
! VF2 iGEM09
+
! VF2 primer
| 1 uL  
| 1 uL  
|-
|-
-
! VR iGEM09
+
! VR primer
| 1 uL  
| 1 uL  
|-
|-
-
! vector
+
! DNA
| 1 uL
| 1 uL
|-
|-
! Taq polymerase
! Taq polymerase
| 1 uL
| 1 uL
 +
|}
 +
 +
|width="10"|
 +
|
 +
{|Border="1"
 +
|
 +
|DNA
 +
|cup nr 1 2 3 4
 +
|cup nr 5 6 7 8
 +
|-
 +
|A
 +
|K077124 in pSB1A2
 +
|VR/VF2 iGEM09
 +
|VR/VF2 iGEM08Aug
 +
|-
 +
|B
 +
|pSB3K3
 +
|VR/VF2 iGEM09
 +
|VR/VF2 iGEM08Aug
 +
|-
 +
|C
 +
|Blanco
 +
|VR/VF2 iGEM09
 +
|VR/VF2 iGEM08Aug
 +
|-
 +
|D
 +
|pUC
 +
|VR/VF2 iGEM09
 +
|VR/VF2 iGEM08Aug
|}
|}
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|}
|}
-
 
-
 
==Dry==
==Dry==
{{Team:Groningen/Notebook/Day/Footer}}
{{Team:Groningen/Notebook/Day/Footer}}

Revision as of 12:07, 23 July 2009

Igemhomelogo.png

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Wet

GVP Cluster

Transporters

Metal Accumulation

Vectors

VR-VF2
Component amount
MasterMix NH4 21 uL
VF2 primer 1 uL
VR primer 1 uL
DNA 1 uL
Taq polymerase 1 uL
DNA cup nr 1 2 3 4 cup nr 5 6 7 8
A K077124 in pSB1A2 VR/VF2 iGEM09 VR/VF2 iGEM08Aug
B pSB3K3 VR/VF2 iGEM09 VR/VF2 iGEM08Aug
C Blanco VR/VF2 iGEM09 VR/VF2 iGEM08Aug
D pUC VR/VF2 iGEM09 VR/VF2 iGEM08Aug


VR VF2 gradient program
Stage Temperature Time
Denaturing 95° 4 min
Start Cycles 30X
Denaturing 95° 1.30 min
Annealing Gradient 50°-62° 30 sec
Elongation 72° 1.30 min
End cycles
Final elongation 72° 6 min
Hold 4° Forever

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
Retrieved from "http://2009.igem.org/Team:Groningen/Notebook/23_July_2009"