Team:Groningen/Notebook/23 July 2009

From 2009.igem.org

(Difference between revisions)
(→Vectors)
(→Vectors)
Line 85: Line 85:
|Denaturing
|Denaturing
|95°
|95°
-
|1.30 min
+
|30 sec
|-
|-
|Annealing
|Annealing
-
|Gradient 50°-62°
+
|Gradient 51°-60°
|30 sec
|30 sec
|-
|-
|Elongation
|Elongation
|72°
|72°
-
|1.30 min
+
|1.50 min
|-
|-
|  
|  
Line 100: Line 100:
|Final elongation  
|Final elongation  
|72°
|72°
-
|6 min
+
|10 min
|-
|-
|Hold
|Hold

Revision as of 12:08, 23 July 2009

Igemhomelogo.png

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Wet

GVP Cluster

Transporters

Metal Accumulation

Vectors

VR-VF2
Component amount
MasterMix NH4 21 uL
VF2 primer 1 uL
VR primer 1 uL
DNA 1 uL
Taq polymerase 1 uL
DNA cup nr 1 2 3 4 cup nr 5 6 7 8
A K077124 in pSB1A2 VR/VF2 iGEM09 VR/VF2 iGEM08Aug
B pSB3K3 VR/VF2 iGEM09 VR/VF2 iGEM08Aug
C Blanco VR/VF2 iGEM09 VR/VF2 iGEM08Aug
D pUC VR/VF2 iGEM09 VR/VF2 iGEM08Aug


VR VF2 gradient program
Stage Temperature Time
Denaturing 95° 4 min
Start Cycles 30X
Denaturing 95° 30 sec
Annealing Gradient 51°-60° 30 sec
Elongation 72° 1.50 min
End cycles
Final elongation 72° 10 min
Hold 4° Forever

Dry

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
Retrieved from "http://2009.igem.org/Team:Groningen/Notebook/23_July_2009"