Team:Groningen/Notebook/23 July 2009

From 2009.igem.org

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==Dry==
==Dry==
As Nienke's preliminary [http://partsregistry.org/Measurement RPU computations] looked promising Jasper started to redo them using more data, and with the goal to ultimately get the results on the Wiki.
As Nienke's preliminary [http://partsregistry.org/Measurement RPU computations] looked promising Jasper started to redo them using more data, and with the goal to ultimately get the results on the Wiki.
 +
 +
KB put the most essential equations of ArsB on the wiki. The afternoon was spent studing more closely the experimental data of the [[http://www.jbc.org/cgi/content/abstract/M400037200v1 paper]] found yesterday.
{{Team:Groningen/Notebook/Day/Footer}}
{{Team:Groningen/Notebook/Day/Footer}}

Revision as of 13:22, 24 July 2009

Igemhomelogo.png

Wet

GVP Cluster

Transporters

Metal Accumulation

Vectors

VR-VF2
Component amount
MasterMix NH4 21 uL
VF2 primer 1 uL
VR primer 1 uL
DNA 1 uL
Taq polymerase 1 uL
DNA and primers
DNA cup nr 1 2 3 4 cup nr 5 6 7 8
A , in , VR/VF2 iGEM09 VR/VF2 iGEM08Aug
B VR/VF2 iGEM09 VR/VF2 iGEM08Aug
C Blanco VR/VF2 iGEM09 VR/VF2 iGEM08Aug
D pUC VR/VF2 iGEM09 VR/VF2 iGEM08Aug


VR VF2 gradient program
Stage Temperature Time
Denaturing 95° 4 min
Start Cycles 30X
Denaturing 95° 30 sec
Annealing Gradient 51°-60° 30 sec
Elongation 72° 1.50 min
End cycles
Final elongation 72° 10 min
Hold Forever

Dry

As Nienke's preliminary RPU computations looked promising Jasper started to redo them using more data, and with the goal to ultimately get the results on the Wiki.

KB put the most essential equations of ArsB on the wiki. The afternoon was spent studing more closely the experimental data of the [paper] found yesterday.


April
MTWTFSS
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6 7 8 9 10 11 12
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20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
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11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November
MTWTFSS
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2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30