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Team:Groningen/Notebook/23 July 2009
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The Project
The Project
WholeSystem
Applications
Transport
Accumulation
Metal-sensitive Promoters
Gas Vesicles
Modelling
Modelling
Detailed model
Characterization
Downloads
HumanPractice
HumanPractice
safety and security
Ethics
Vision
Survey
Biobricks
Biobricks
Our registry
Submitted Biobricks
Used Biobricks
Project Plan
UPEDU
Project Plan
Brainstorm
Future
Risklist
The Team
The Team
Pictures
Videos
contact
Acknowledgements
Notebook
April
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May
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June
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July
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August
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September
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October
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November
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Glossary
Literature
[1]
Protocols
Wet
GVP Cluster
Transporters
Metal Accumulation
Vectors
VR-VF2
Component
amount
MasterMix NH4
21 uL
VF2 primer
1 uL
VR primer
1 uL
DNA
1 uL
Taq polymerase
1 uL
DNA
cup nr 1 2 3 4
cup nr 5 6 7 8
A
K077124 in pSB1A2
VR/VF2 iGEM09
VR/VF2 iGEM08Aug
B
pSB3K3
VR/VF2 iGEM09
VR/VF2 iGEM08Aug
C
Blanco
VR/VF2 iGEM09
VR/VF2 iGEM08Aug
D
pUC
VR/VF2 iGEM09
VR/VF2 iGEM08Aug
VR VF2 gradient program
Stage
Temperature
Time
Denaturing
95°
4 min
Start Cycles 30X
Denaturing
95°
1.30 min
Annealing
Gradient 50°-62°
30 sec
Elongation
72°
1.30 min
End cycles
Final elongation
72°
6 min
Hold
4°
Forever
Dry
April
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May
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June
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July
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August
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September
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October
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November
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