Team:Groningen/Notebook/24 August 2009
From 2009.igem.org
(Difference between revisions)
(→Vectors) |
(→Metal promotors) |
||
Line 20: | Line 20: | ||
===Vectors=== | ===Vectors=== | ||
====Metal promotors==== | ====Metal promotors==== | ||
- | + | [http://wolfson.huji.ac.il/expression/vector/origin.html#top origin of replication] determines: | |
- | * | + | * Vector copy number |
- | * | + | * Plasmid compatibility: its ability to replicate in conjuction with another plasmid. |
+ | ** Plasmids that utilize the same replication system cannot co-exist in the same bacterial cell. | ||
+ | ** pUC19 and ColE1 are in different [http://wolfson.huji.ac.il/expression/vector/origin.html#comp compatibility group] | ||
==Dry== | ==Dry== | ||
{{Team:Groningen/Notebook/Day/Footer}} | {{Team:Groningen/Notebook/Day/Footer}} |
Revision as of 13:26, 24 August 2009
Wet
GVP Cluster
- → TODO Restriction of GVP and pSB1AC3-Lac/pBAD for assembly
- → TODO Gel purification of wanted fragments and nanodrop
- → TODO Clone GVP cluster behind Lac and Arabinose promoter for expression tests in pSB1AC3 plasmid
- → TODO Send plasmids with MEtal promoters in BBa_J61002 vector for sequencing
- → TODO Check MEtal + RBS promoters on gel with restriction (also XbaI/PstI in Zinc promoter)
- → TODO Test promoter strenght compared to BBa_J23101 promoter (Sven)
- → TODO Enter sequences of constructs to Sandbox
Transporters
Metal Accumulation
Vectors
Metal promotors
origin of replication determines:
- Vector copy number
- Plasmid compatibility: its ability to replicate in conjuction with another plasmid.
- Plasmids that utilize the same replication system cannot co-exist in the same bacterial cell.
- pUC19 and ColE1 are in different compatibility group
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|