Team:Groningen/Parts

From 2009.igem.org

(Difference between revisions)
m
Line 45: Line 45:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 54: Line 54:
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 63: Line 63:
|Low consitutive promoter
|Low consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
-
|35bp
+
|35
|<partinfo>pSB3K3</partinfo>  
|<partinfo>pSB3K3</partinfo>  
|Kanamycin
|Kanamycin
Line 72: Line 72:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23100</partinfo>
|<partinfo>J23100</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 81: Line 81:
|Medium consitutive promoter
|Medium consitutive promoter
|<partinfo>J23106</partinfo>
|<partinfo>J23106</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 90: Line 90:
|High consitutive promoter
|High consitutive promoter
|<partinfo>J23109</partinfo>
|<partinfo>J23109</partinfo>
-
|35bp
+
|35
|<partinfo>pSB1AC3</partinfo>  
|<partinfo>pSB1AC3</partinfo>  
|Ampicillin/Chloramphenicol
|Ampicillin/Chloramphenicol
Line 126: Line 126:
|Lactose inducible promoter nr1
|Lactose inducible promoter nr1
|<partinfo>R0010</partinfo>
|<partinfo>R0010</partinfo>
-
|200bp
+
|200
|<partinfo>pSB1A2</partinfo>  
|<partinfo>pSB1A2</partinfo>  
|Ampicillin
|Ampicillin
Line 135: Line 135:
|Lactose inducible promoter nr2
|Lactose inducible promoter nr2
|<partinfo>R0010</partinfo>
|<partinfo>R0010</partinfo>
-
|200bp
+
|200
|<partinfo>pSB1A2</partinfo>  
|<partinfo>pSB1A2</partinfo>  
|Ampicillin
|Ampicillin
Line 289: Line 289:
|<partinfo>BBa_K190024</partinfo>
|<partinfo>BBa_K190024</partinfo>
|71
|71
-
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61002 BBa_J61002] (without the part between EcoRI and SpeI)
+
|<partinfo>BBa_J61002</partinfo> (without the part between EcoRI and SpeI)
|Ampicillin
|Ampicillin
|plate 3, pos.45
|plate 3, pos.45
Line 297: Line 297:
|SmtA-GST fusion  
|SmtA-GST fusion  
|no biobrick primers available
|no biobrick primers available
-
|1400bp
+
|1400
-
|pGEX-3x (NEB)
+
|[http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x]
|Ampicillin
|Ampicillin
|plate 3, pos. 46
|plate 3, pos. 46
Line 306: Line 306:
|SmtA
|SmtA
|<partinfo>BBa_K190021</partinfo>
|<partinfo>BBa_K190021</partinfo>
-
|900bp
+
|900
-
|pET29a (NEB)
+
|[http://www.merckbiosciences.co.uk/product/69871 pET29a]
|Kanamycin
|Kanamycin
|plate 3, pos. 47
|plate 3, pos. 47
Line 315: Line 315:
|MymT
|MymT
|<partinfo>BBa_K190020</partinfo>
|<partinfo>BBa_K190020</partinfo>
-
|215bp
+
|215
 +
|[http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB)
|Ampicillin
|Ampicillin
-
|pTXB1 (NEB)
 
|plate 3, pos. 48
|plate 3, pos. 48
|PCR, restriction
|PCR, restriction
Line 324: Line 324:
|GlpF and fMT on a synthetic operon in the vector
|GlpF and fMT on a synthetic operon in the vector
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
|<partinfo>BBa_K190019</partinfo> and <partinfo>BBa_K190028</partinfo>
-
|fMT = 200bp, GlpF = 900bp?
+
|fMT = 200, GlpF = 900?
 +
|[http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB)
|Ampicillin
|Ampicillin
-
|pMALc2x (NEB)
 
|plate 3, pos. 49
|plate 3, pos. 49
|PCR, restriction
|PCR, restriction
Line 333: Line 333:
|MBP-ArsR Fusion protein
|MBP-ArsR Fusion protein
|<partinfo>BBa_K190027</partinfo>
|<partinfo>BBa_K190027</partinfo>
-
|15180 bp
+
|15180
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 342: Line 342:
|GVP Gene cluster
|GVP Gene cluster
|{{todo|}}
|{{todo|}}
-
|6000 bp
+
|6000
-
|pBluescriptIIKS
+
|[http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS]
|{{todo| Ampicillin}}
|{{todo| Ampicillin}}
|plate 3, pos. 54
|plate 3, pos. 54
Line 351: Line 351:
|Metallothionein with inducible promotor
|Metallothionein with inducible promotor
|<partinfo>BBa_K190032</partinfo>
|<partinfo>BBa_K190032</partinfo>
-
|430 bp
+
|430
|<partinfo>pSB1A2</partinfo>
|<partinfo>pSB1A2</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 360: Line 360:
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
|<partinfo>BBa_K190031</partinfo>
|<partinfo>BBa_K190031</partinfo>
-
|265 bp
+
|265
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 369: Line 369:
|Metallothionein with low constitutive promotor
|Metallothionein with low constitutive promotor
|<partinfo>BBa_K190031</partinfo>
|<partinfo>BBa_K190031</partinfo>
-
|265 bp
+
|265  
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 378: Line 378:
|Metallothionein
|Metallothionein
|<partinfo>BBa_K190021</partinfo>
|<partinfo>BBa_K190021</partinfo>
-
|678 bp
+
|678
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 387: Line 387:
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
|<partinfo>BBa_K190019</partinfo>
|<partinfo>BBa_K190019</partinfo>
-
|222 bp
+
|222
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol
Line 396: Line 396:
|Metallothionein for As<sup>3+</sup>
|Metallothionein for As<sup>3+</sup>
|<partinfo>BBa_K190019</partinfo>
|<partinfo>BBa_K190019</partinfo>
-
| 222 bp
+
| 222
|<partinfo>pSB1AC3</partinfo>
|<partinfo>pSB1AC3</partinfo>
|Ampicillin/chloramphenicol
|Ampicillin/chloramphenicol

Revision as of 08:31, 4 September 2009

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Biobricks


Note

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Part List -80

Component Description Part or Accession # Base Pairs (bp) Plasmid (backbone) Resistance Well Quality control
GVP Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.1 ?
GVP (2nd batch) Gas Vesicle Proteins BBa_I750016 6064 BBa_J61035 Ampicillin/Gentamycin plate 3, pos.2 ?
pHigh in low copy nr vector High consitutive promoter BBa_J23100 35 pSB3K3 Kanamycin Plate 3, pos. 4 PCR(?), restriction(+)
pMed in low copy nr vector Medium consitutive promoter BBa_J23106 35 pSB3K3 Kanamycin Plate 3, pos. 5 PCR(?), restriction(+)
pLow in low copy nr vector Low consitutive promoter BBa_J23109 35 pSB3K3 Kanamycin Plate 3, pos. 6 PCR(?), restriction(+)
pHigh in high copy nr vector High consitutive promoter BBa_J23100 35 pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 7 PCR(?), restriction(+)
pMed in high copy nr vector Medium consitutive promoter BBa_J23106 35 pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 8 PCR(?), restriction(+)
pLow in high copy nr vector High consitutive promoter BBa_J23109 35 pSB1AC3 Ampicillin/Chloramphenicol Plate 3, pos. 9 PCR(?), restriction(+)
Promotor (low expression) Constitutive promotor BBa_J23109 35 BBa_J61002 Ampicillin plate 3, pos.10 No
Promotor (medium expression) Constitutive promotor BBa_J23106 35 BBa_J61002 Ampicillin plate 3, pos.11 No
Promotor (high expression) Constitutive promotor BBa_J23100 35 BBa_J61002 Ampicillin plate 3, pos.12 No
Inducible promoter Lactose inducible promoter nr1 BBa_R0010 200 pSB1A2 Ampicillin Plate 3, pos. 14 PCR(?), restriction(+)
Inducible promoter Lactose inducible promoter nr2 BBa_R0010 200 pSB1A2 Ampicillin Plate 3, pos. 15 PCR(?), restriction(+)
RBS Ribosomal Binding Site BBa_B0034 12 pSB1A2 Ampicillin plate 3, pos.19 No
Terminator Double terminator consisting of BBa_B0012 and BBa_B0011] BBa_B0014 ~90 pSB1AK3 Ampicillin/Kanamycin plate 3, pos.20 No
Const. Pormoter + GVP Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.21 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Low constitutive promoter with GVP cluster BBa_J23109 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.22 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.23 EcoRI/PstI + Sequenced Okay
Const. Pormoter + GVP (no.2) Medium constitutive promoter with GVP cluster BBa_J23106 + BBa_I750016 ~6100 pSB1AC3 Ampicillin/Chloramphenicol plate 3, pos.24 EcoRI/PstI + Sequenced Okay
HmtA (1.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.28 Restriction(+)
HmtA (2.1) HmtA-His in pBAD(a) None ? None Ampicillin plate 3, pos.29 Restriction(+)
CueO- + GVP in J61035 CueO promoter without RBS in front of the GVP cluster None 6149 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.37 Restriction(+) + Sequenced Okay
ZntR- + GVP in J61035 ZntR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.38 Restriction(+) + Sequenced (3 mutations)
ArsR- + GVP in J61035 ArsR promoter without RBS in front of the GVP cluster None ~6150 BBa_J61035 (without the part between EcoRI and XbaI) Ampicillin plate 3, pos.39 Restriction(+) + Sequenced Okay
ArsR- in J61002 pArsR (promoter without RBS) BBa_K190015 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.40 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR- in J61002 pZntR (promoter without RBS) BBa_K190016 65 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.41 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
CueO- in J61002 pCueO (promoter without RBS) BBa_K190017 43 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.42 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ZntR+RBS in J61002 pZntR (promoter with own RBS) BBa_K190022 91 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.43 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
ArsR+RBS in J61002 pArsR (promoter with own RBS) BBa_K190023 81 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.44 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
CueO+RBS in J61002 pCueO (promoter with own RBS) BBa_K190024 71 BBa_J61002 (without the part between EcoRI and SpeI) Ampicillin plate 3, pos.45 EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
SmtA-GST in pGEX-3x SmtA-GST fusion no biobrick primers available 1400 pGEX-3x Ampicillin plate 3, pos. 46 PCR, restriction
SmtA in pET29a SmtA BBa_K190021 900 pET29a Kanamycin plate 3, pos. 47 PCR, restriction
MymT in pGB68 MymT BBa_K190020 215 pTXB1 (NEB) Ampicillin plate 3, pos. 48 PCR, restriction
GlpF and fMT in pMAL-MT GlpF and fMT on a synthetic operon in the vector BBa_K190019 and BBa_K190028 fMT = 200, GlpF = 900? pMALc2x (NEB) Ampicillin plate 3, pos. 49 PCR, restriction
MBP-ArsR MBP-ArsR Fusion protein BBa_K190027 15180 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 53 Restriction analysis] & PCR
pNL29 GVP Gene cluster 6000 pBluescriptIIKS Ampicillin plate 3, pos. 54 Buoyancy phenotype
pLac-fMT Metallothionein with inducible promotor BBa_K190032 430 pSB1A2 Ampicillin/chloramphenicol plate 3, pos. 55 Quality control
pLow-fMT #4 Metallothionein with low constitutive promotor BBa_K190031 265 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 56 Quality control
pLow-fMT #3 Metallothionein with low constitutive promotor BBa_K190031 265 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 57 Quality control
SmtA Metallothionein BBa_K190021 678 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 58 Quality control
fMT #6 Metallothionein for As3+ BBa_K190019 222 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 59 Sequence 2 deletions between EcoRI and XbaI sites
fMT #4 Metallothionein for As3+ BBa_K190019 222 pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 60 Sequence Okay!
High consitutive promoter(with RFP) Constitutive promoter BBa_J23100 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 75 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter BBa_J23106 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 76 Quality control
Low consitutive promoter(with RFP) Constitutive promoter BBa_J23109 Base Pairs (bp) pSB1AC3 Ampicillin/chloramphenicol plate 3, pos. 77 Quality control
High consitutive promoter(with RFP) Constitutive promoter BBa_J23100 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 78 Quality control
Medium consitutive promoter(with RFP) Constitutive promoter BBa_J23106 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 79 Quality control
Low consitutive promoter(with RFP) Constitutive promoter BBa_J23109 Base Pairs (bp) pSB3K3 Kanamycin plate 3, pos. 80 Quality control
Reference constitutive promoter) Constitutive promoter ? Base Pairs (bp) ? ? plate 3, pos. 81 Quality control