Team:Groningen/Parts
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Biobricks
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Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
Our sandbox
Part List -80
Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
GVP | Gas Vesicle Proteins | BBa_I750016 | 6064 | BBa_J61035 | Ampicillin/Gentamycin | plate 3, pos.1 | ? |
GVP (2nd batch) | Gas Vesicle Proteins | BBa_I750016 | 6064 | BBa_J61035 | Ampicillin/Gentamycin | plate 3, pos.2 | ? |
pHigh in low copy nr vector | High consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
pMed in low copy nr vector | Medium consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
pLow in low copy nr vector | Low consitutive promoter | 35bp | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
pHigh in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
pMed in high copy nr vector | Medium consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
pLow in high copy nr vector | High consitutive promoter | 35bp | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
Promotor (low expression) | Constitutive promotor | BBa_J23109 | 35 | BBa_J61002 | Ampicillin | plate 3, pos.10 | No |
Promotor (medium expression) | Constitutive promotor | BBa_J23106 | 35 | BBa_J61002 | Ampicillin | plate 3, pos.11 | No |
Promotor (high expression) | Constitutive promotor | BBa_J23100 | 35 | BBa_J61002 | Ampicillin | plate 3, pos.12 | No |
Inducible promoter | Lactose inducible promoter nr1 | 200bp | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
Inducible promoter | Lactose inducible promoter nr2 | 200bp | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
RBS | Ribosomal Binding Site | BBa_B0034 | 12 | pSB1A2 | Ampicillin | plate 3, pos.19 | No |
Terminator | Double terminator consisting of BBa_B0012 and BBa_B0011 | BBa_B0014 | ~90 | pSB1AK3 | Ampicillin/Kanamycin | plate 3, pos.20 | No |
HmtA (1.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.28 | Restriction(+) |
HmtA (2.1) | HmtA-His in pBAD(a) | None | ? | None | Ampicillin | plate 3, pos.29 | Restriction(+) |
CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | BBa_J61035 (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) |
ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | BBa_J61035 (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) |
ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | BBa_J61035 (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) |