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Team:TUDelft/7 August 2009
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| - | The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS). | + | The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS). Should be able to save some assembly steps by using this part |
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Revision as of 12:01, 19 October 2009
Lab Notebook
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7 August 2009
Tim Weenink
Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.
Calin
Designed short knockout primers. CA had colonies. But not all expressed GFP:
| Set | GFP colonies | Non-GFP colonies |
| RbCl | 3 | 140 |
| TSS | 1? | 7 |
| ElectroPoration | 6 | 54 |
The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS). Should be able to save some assembly steps by using this part
| Set | Time Constant |
| Assembly 1 | 4.4 |
| BBa_J13002 | 4.6 |
Daniel made CAM control plates at both high and low concentrations.
