Team:Paris/Freezer Digestion Products

From 2009.igem.org

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{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
==Freezer==
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<html>
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<style type="text/css">
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#left-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    top: 0px;
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    left: 10px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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#middle-side {
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    height: 25px;
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    width: 650px;
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    position: absolute;
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    top: 0px;
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    left: 30px;
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    margin-top:10px;
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    padding-top: 5px;
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    background: #dadada;
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    z-index:5;
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}
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#right-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    margin-top:10px;
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    padding-top: 7px;
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    top: 0px;
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    left: 670px;
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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a.menu_sub {
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    padding-left: 7px;
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    padding-right: 7px;
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}
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a.menu_sub_active {
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    padding-left: 7px;
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    padding-right: 7px;
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    color:#b0310e;
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    font-weight:bold;
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}
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
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</center>
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</div>
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<div id="right-side"></div>
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</html>
<center>
<center>
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -  
[[Team:Paris/Freezer_Primers#Freezer | Primers]] -  

Revision as of 15:22, 19 October 2009

iGEM > Paris > Freezer > Digestion Products


Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size Yes Yes 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size Yes Yes 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size Yes Yes 08/07
D10 ptet  ??? P18 XbaI PstI Size Yes Yes 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes Yes 08/10
D13 OmpAsignal  ??? A11 XbaI PstI ~135 Yes Yes 11/07
D14 TolRII  ??? A13 XbaI PstI ~279 Yes Yes 11/07
D15 double terminator [P4]BBa-B0014 P4 EcoRI SpeI 95pb Yes  ??? 11/08
D16 pLac [P21]BBa-I1014 P21 EcoRI XbaI  ??? Yes  ??? 11/08

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Digestion_Products"