Team:Paris/7 August 2009
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*All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority. | *All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority. | ||
+ | </div> | ||
+ | |||
+ | <div class="guillaume"> | ||
+ | Miniprep | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | P15, P16, P17, P18, P19 | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Digestion | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4) | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Migration | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | GEL | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Purification | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | valeur | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Verification sur gel | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | GEL | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | ON culture | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | P1, P2, P17, S31 | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Overnight PCR | ||
+ | </div> | ||
+ | <div class="experince"> | ||
+ | compo | ||
</div> | </div> | ||
<html> | <html> |
Revision as of 23:43, 7 August 2009
Contents |
NoteBook
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Lab work
Microscope
Microscopy
We try using the time laps with two filters. cf protocol
Molecular Biology
Reception of primers (oligos)
- Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.
- All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.
Miniprep
P15, P16, P17, P18, P19
Digestion
P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4)
Migration
GEL
Purification
valeur
Verification sur gel
GEL
ON culture
P1, P2, P17, S31
Overnight PCR
compo
To do list
Matricule | TODO |
Luc | microscope |
Romain | walking / wiki |
Charlotte | Jun/Fos system |
Stoff | Wiki update / Nair-tree protocol algorithm |
Chris | home sweet home ... but still looking for one |
Lisa | should be back |
Caroline | microscope |
Souf | |
Vicard | Lab |
Pierre | microscope / Modeling |
Sylvain | Run to Necker Hospital and come back with the primers. PCR |
Guillaume | lab |