Team:Paris/7 August 2009
From 2009.igem.org
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Revision as of 23:44, 7 August 2009
Contents |
NoteBook
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Lab work
Microscope
Microscopy
We try using the time laps with two filters. cf protocol
Molecular Biology
Reception of primers (oligos)
- Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.
- All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.
Miniprep
P15, P16, P17, P18, P19
Digestion
P15, P16, P18, P19 by XbaI,PstI (Neb2), P17 by EcoRI,SpeI (Neb4)
Migration
GEL
Purification
valeur
Verification sur gel
GEL
ON culture
P1, P2, P17, S31
Overnight PCR
compo
To do list
Matricule | TODO |
Luc | microscope |
Romain | walking / wiki |
Charlotte | Jun/Fos system |
Stoff | Wiki update / Nair-tree protocol algorithm |
Chris | home sweet home ... but still looking for one |
Lisa | should be back |
Caroline | microscope |
Souf | |
Vicard | Lab |
Pierre | microscope / Modeling |
Sylvain | Run to Necker Hospital and come back with the primers. PCR |
Guillaume | lab |