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Team:Paris/Freezer Plasmids
From 2009.igem.org
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|width=5%| Date | |width=5%| Date | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P1 | + | |width=5%| P1 <span/ id="P1"> |
|width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
|width=50%| General plasmid for cloning step after PCR | |width=50%| General plasmid for cloning step after PCR | ||
| Line 50: | Line 50: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P2 | + | |width=5%| P2 <span/ id="P2"> |
|width=25%| [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | |width=25%| [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | ||
|width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter | |width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter | ||
| Line 56: | Line 56: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P3 | + | |width=5%| P3 <span/ id="P3"> |
|width=25%| [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] | |width=25%| [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] | ||
|width=50%| PelB leader sequence | |width=50%| PelB leader sequence | ||
| Line 62: | Line 62: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P4 | + | |width=5%| P4 <span/ id="P4"> |
|width=25%| [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] | |width=25%| [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] | ||
|width=50%| Double terminator bidirectionnal (B0012-B0011) | |width=50%| Double terminator bidirectionnal (B0012-B0011) | ||
| Line 68: | Line 68: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P5 | + | |width=5%| P5 <span/ id="P5"> |
|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] | |width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] | ||
|width=50%| pLac | |width=50%| pLac | ||
| Line 74: | Line 74: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P6 | + | |width=5%| P6 <span/ id="P6"> |
|width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] | |width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] | ||
|width=50%| OmpA linker (464bp) | |width=50%| OmpA linker (464bp) | ||
| Line 80: | Line 80: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P7 | + | |width=5%| P7 <span/ id="P7"> |
|width=25%| [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] | |width=25%| [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] | ||
|width=50%| TetR repressible promoter | |width=50%| TetR repressible promoter | ||
| Line 86: | Line 86: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P8 | + | |width=5%| P8 <span/ id="P8"> |
|width=25%| [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] | |width=25%| [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] | ||
|width=50%| pBad/araC | |width=50%| pBad/araC | ||
| Line 92: | Line 92: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P9 | + | |width=5%| P9 <span/ id="P9"> |
|width=25%| ΔN-Syn1A | |width=25%| ΔN-Syn1A | ||
|width=50%| ??? | |width=50%| ??? | ||
| Line 98: | Line 98: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P10 | + | |width=5%| P10 <span/ id="P10"> |
|width=25%| His6-SNAP25 | |width=25%| His6-SNAP25 | ||
|width=50%| ??? | |width=50%| ??? | ||
| Line 104: | Line 104: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P11 | + | |width=5%| P11 <span/ id="P11"> |
|width=25%| VAMP2-HiB6 | |width=25%| VAMP2-HiB6 | ||
|width=50%| ??? | |width=50%| ??? | ||
| Line 110: | Line 110: | ||
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P12 | + | |width=5%| P12 <span/ id="P12"> |
|width=25%| g3p | |width=25%| g3p | ||
|width=50%| g3p in pHEN2 plasmid | |width=50%| g3p in pHEN2 plasmid | ||
| Line 116: | Line 116: | ||
|width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | |width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P13 | + | |width=5%| P13 <span/ id="P13"> |
|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3] | |width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
|width=50%| High copy BioBrick assembly plasmid | |width=50%| High copy BioBrick assembly plasmid | ||
| Line 122: | Line 122: | ||
|width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06] | |width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P14 | + | |width=5%| P14 <span/ id="P14"> |
|width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | |width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | ||
|width=50%| RBS-tetR | |width=50%| RBS-tetR | ||
| Line 128: | Line 128: | ||
|width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06] | |width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P15 | + | |width=5%| P15 <span/ id="P15"> |
|width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
|width=50%| plasmid backbone | |width=50%| plasmid backbone | ||
| Line 134: | Line 134: | ||
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | |width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P16 | + | |width=5%| P16 <span/ id="P16"> |
|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3] | |width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ||
|width=50%| plasmid backbone | |width=50%| plasmid backbone | ||
| Line 140: | Line 140: | ||
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | |width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P17 | + | |width=5%| P17 <span/ id="P17"> |
|width=25%| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | |width=25%| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | ||
|width=50%| plasmid backbone with RFP | |width=50%| plasmid backbone with RFP | ||
| Line 146: | Line 146: | ||
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | |width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P18 | + | |width=5%| P18 <span/ id="P18"> |
|width=25%| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | |width=25%| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | ||
|width=50%| ptet | |width=50%| ptet | ||
| Line 152: | Line 152: | ||
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | |width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P19 | + | |width=5%| P19 <span/ id="P19"> |
|width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | |width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | ||
|width=50%| RBS-tet | |width=50%| RBS-tet | ||
| Line 158: | Line 158: | ||
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | |width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
| - | |width=5%| P20 | + | |width=5%| P20 <span/ id="P20"> |
|width=25%| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | |width=25%| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | ||
|width=50%| RBS-lacI | |width=50%| RBS-lacI | ||
Revision as of 13:24, 10 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Plasmids
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| P1 | [http://partsregistry.org/Part:pSB2K3 pSB2K3] | General plasmid for cloning step after PCR | Products in use | 08/03 |
| P2 | [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | Plasmid backbone, it allows us to change promoter RFP as promoter | Products in use | 08/03 |
| P3 | [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] | PelB leader sequence | Products in use | 08/03 |
| P4 | [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] | Double terminator bidirectionnal (B0012-B0011) | Products in use | 08/03 |
| P5 | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] | pLac | Products in use | 08/03 |
| P6 | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] | OmpA linker (464bp) | Products in use | 08/03 |
| P7 | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] | TetR repressible promoter | Products in use | 08/03 |
| P8 | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] | pBad/araC | Products in use | 08/03 |
| P9 | ΔN-Syn1A | ??? | Products in use | 08/03 |
| P10 | His6-SNAP25 | ??? | Products in use | 08/03 |
| P11 | VAMP2-HiB6 | ??? | Products in use | 08/03 |
| P12 | g3p | g3p in pHEN2 plasmid | Products in use | 08/04 |
| P13 | [http://partsregistry.org/Part:pSB1A3 pSB1A3] | High copy BioBrick assembly plasmid | Products in use | 08/06 |
| P14 | [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | RBS-tetR | Products in use | 08/06 |
| P15 | [http://partsregistry.org/Part:pSB2K3 pSB2K3] | plasmid backbone | All have been digested and purified | 08/07 |
| P16 | [http://partsregistry.org/Part:pSB1A3 pSB1A3] | plasmid backbone | All have been digested and purified | 08/07 |
| P17 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | plasmid backbone with RFP | All have been digested and purified | 08/07 |
| P18 | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | ptet | All have been digested and purified | 08/07 |
| P19 | [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | RBS-tet | All have been digested and purified | 08/07 |
| P20 | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS-lacI | ... | 08/08 |
