Team:Paris/Freezer Digestion Products

From 2009.igem.org

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Revision as of 20:01, 10 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 P15  ??? XbaI PstI Size  ???  ???  ??? 08/07
D8 P16  ??? XbaI PstI Size  ???  ???  ??? 08/07
D9 P17  ??? EcoRI SpeI Size  ???  ???  ??? 08/07
D10 P18  ??? XbaI PstI Size  ???  ???  ??? 08/07
D11 P19  ??? XbaI PstI Size  ???  ???  ??? 08/07
D12 P21  ??? SpeI PstI Size  ???  ???  ??? 08/07

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Digestion_Products"