Team:Paris/Freezer Digestion Products
From 2009.igem.org
(Difference between revisions)
(→Digestion Products) |
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|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D7 | |style="background:#FFFF00"| D7 | ||
- | | | + | | pSB2K3 |
| ??? | | ??? | ||
+ | | P15 | ||
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Size | | Size | ||
- | |||
| ??? | | ??? | ||
| ??? | | ??? | ||
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|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D8 | |style="background:#FFFF00"| D8 | ||
- | | | + | | pSB1A3 |
| ??? | | ??? | ||
+ | | P16 | ||
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Size | | Size | ||
- | |||
| ??? | | ??? | ||
| ??? | | ??? | ||
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|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D9 | |style="background:#FFFF00"| D9 | ||
- | | | + | | plbb RFP |
| ??? | | ??? | ||
+ | | P17 | ||
| EcoRI | | EcoRI | ||
| SpeI | | SpeI | ||
| Size | | Size | ||
- | |||
| ??? | | ??? | ||
| ??? | | ??? | ||
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|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D10 | |style="background:#FFFF00"| D10 | ||
+ | | ptet | ||
+ | | ??? | ||
| P18 | | P18 | ||
- | |||
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Size | | Size | ||
- | |||
| ??? | | ??? | ||
| ??? | | ??? | ||
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|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D11 | |style="background:#FFFF00"| D11 | ||
- | | | + | | RBS-tet |
| ??? | | ??? | ||
+ | | P19 | ||
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Size | | Size | ||
- | | | + | | Yes |
- | | | + | | Yes |
- | + | ||
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | | [https://2009.igem.org/Team:Paris/7_August_2009 08/07] | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D12 | |style="background:#FFFF00"| D12 | ||
+ | | pLac | ||
+ | | In order to open the vector to add RBS-tet (XbaI/PstI) | ||
| P21 | | P21 | ||
- | |||
| SpeI | | SpeI | ||
| PstI | | PstI | ||
| Size | | Size | ||
- | | | + | | Yes |
- | | | + | | No |
- | + | | [https://2009.igem.org/Team:Paris/10_August_2009 08/10] | |
- | | [https://2009.igem.org/Team:Paris/ | + | |
|} | |} |
Revision as of 20:11, 10 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
Digestion Products | |||||||||
---|---|---|---|---|---|---|---|---|---|
Number | Name | Description | Matrix ID | Enz. 1 | Enz. 2 | Size (bp) | Digested? | Purified? | [ng/µL] |
D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | 66pb | PelB leader sequence | 47 | ??? | 08/04 |
D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | 95pb | Double terminator bidirectionnal (B0012-B0011) | 47 | ??? | 08/04 |
D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | 37pb | pLac | 47 | ??? | 08/04 |
D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | 54pb | TetR repressible promote | 47 | ??? | 08/04 |
D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | 1210pb | pBad/araC | 47 | ??? | 08/04 |
D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | 47 | ??? | 08/04 |
D7 | pSB2K3 | ??? | P15 | XbaI | PstI | Size | ??? | ??? | 08/07 |
D8 | pSB1A3 | ??? | P16 | XbaI | PstI | Size | ??? | ??? | 08/07 |
D9 | plbb RFP | ??? | P17 | EcoRI | SpeI | Size | ??? | ??? | 08/07 |
D10 | ptet | ??? | P18 | XbaI | PstI | Size | ??? | ??? | 08/07 |
D11 | RBS-tet | ??? | P19 | XbaI | PstI | Size | Yes | Yes | 08/07 |
D12 | pLac | In order to open the vector to add RBS-tet (XbaI/PstI) | P21 | SpeI | PstI | Size | Yes | No | 08/10 |