Team:Paris/7 August 2009
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{{Paris2009_Calendar_Link|6_August_2009|8_August_2009}} | {{Paris2009_Calendar_Link|6_August_2009|8_August_2009}} | ||
<center> '''August 7th''' </center> | <center> '''August 7th''' </center> | ||
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===Lab work=== | ===Lab work=== | ||
- | <div | + | ====Microscope==== |
+ | <div class="caroline"> | ||
Microscopy | Microscopy | ||
- | </div><div | + | </div> |
- | We try using the time laps with two filters | + | <div class ="experience">We try using the time laps with [[Team:Paris/ProtocolsA#Protocols suggested | two filters]] |
- | + | ||
</div> | </div> | ||
- | <div | + | ==== Molecular Biology==== |
+ | <div class="sylvain"> | ||
Reception of primers (oligos) | Reception of primers (oligos) | ||
- | </div><div | + | </div> |
- | Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 | + | <div class="experience"> |
- | + | *Primers from 08/03 arrived at Necker. [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 References] : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30. | |
- | + | <br> | |
- | + | *All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority. | |
- | + | </div> | |
- | + | ||
- | All primers received by now are labeled and | + | |
+ | <div class="guillaume"> | ||
+ | Miniprep | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | P15, P16, P17, P18, P19 | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Digestion | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4) | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Migration | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | <center>[[Image:0708_wiki_digestion_P18XP.png]]</center> | ||
+ | <center>[[Image:0708_wiki_digestion_P18XP_extract.png]]</center> | ||
+ | <center>[[Image:0708_wiki_digestion_P15161719.png]]</center> | ||
+ | <center>[[Image:0708_wiki_digestion_P15161719_extract.png]]</center> | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Purification | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | valeur | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Verification sur gel | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | <center>[[Image:0708_wiki_purif_P19.png]]</center> | ||
+ | <center>[[Image:0708_wiki_purif_P15161718.png]]</center> | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | ON culture | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | P1, P2, P17, S31 | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | Overnight PCR | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | compo | ||
</div> | </div> | ||
<html> | <html> |
Latest revision as of 00:31, 11 August 2009
Contents |
NoteBook
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Lab work
Microscope
Microscopy
We try using the time laps with two filters
Molecular Biology
Reception of primers (oligos)
- Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.
- All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.
Miniprep
P15, P16, P17, P18, P19
Digestion
P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4)
Migration
Purification
valeur
Verification sur gel
ON culture
P1, P2, P17, S31
Overnight PCR
compo
To do list
Matricule | TODO |
Luc | microscope |
Romain | walking / wiki |
Charlotte | Jun/Fos system |
Stoff | Wiki update / Nair-tree protocol algorithm |
Chris | home sweet home ... but still looking for one |
Lisa | should be back |
Caroline | microscope |
Souf | |
Vicard | Lab |
Pierre | microscope / Modeling |
Sylvain | Run to Necker Hospital and come back with the primers. PCR |
Guillaume | lab |