"
Team:Paris/7 August 2009
From 2009.igem.org
(Difference between revisions)
(→Molecular Biology) |
(→Molecular Biology) |
||
| (6 intermediate revisions not shown) | |||
| Line 20: | Line 20: | ||
Microscopy | Microscopy | ||
</div> | </div> | ||
| - | <div class ="experience">We try using the time laps with two filters | + | <div class ="experience">We try using the time laps with [[Team:Paris/ProtocolsA#Protocols suggested | two filters]] |
</div> | </div> | ||
| + | |||
==== Molecular Biology==== | ==== Molecular Biology==== | ||
<div class="sylvain"> | <div class="sylvain"> | ||
| Line 27: | Line 28: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
| - | *Primers from 08/03 arrived at Necker. [https://2009.igem.org/Team:Paris/Freezer_Primers References] : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30. | + | *Primers from 08/03 arrived at Necker. [https://2009.igem.org/Team:Paris/Freezer_Primers#O19 References] : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30. |
<br> | <br> | ||
*All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority. | *All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority. | ||
| Line 48: | Line 49: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
| - | + | <center>[[Image:0708_wiki_digestion_P18XP.png]]</center> | |
| + | <center>[[Image:0708_wiki_digestion_P18XP_extract.png]]</center> | ||
| + | <center>[[Image:0708_wiki_digestion_P15161719.png]]</center> | ||
| + | <center>[[Image:0708_wiki_digestion_P15161719_extract.png]]</center> | ||
</div> | </div> | ||
<div class="guillaume"> | <div class="guillaume"> | ||
| Line 60: | Line 64: | ||
</div> | </div> | ||
<div class="experience"> | <div class="experience"> | ||
| - | + | <center>[[Image:0708_wiki_purif_P19.png]]</center> | |
| + | <center>[[Image:0708_wiki_purif_P15161718.png]]</center> | ||
</div> | </div> | ||
<div class="guillaume"> | <div class="guillaume"> | ||
Latest revision as of 00:31, 11 August 2009
Contents |
NoteBook
|
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lab work
Microscope
Microscopy
We try using the time laps with two filters
Molecular Biology
Reception of primers (oligos)
- Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.
- All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.
Miniprep
P15, P16, P17, P18, P19
Digestion
P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4)
Migration
Purification
valeur
Verification sur gel
ON culture
P1, P2, P17, S31
Overnight PCR
compo
To do list
| Matricule | TODO |
| Luc | microscope |
| Romain | walking / wiki |
| Charlotte | Jun/Fos system |
| Stoff | Wiki update / Nair-tree protocol algorithm |
| Chris | home sweet home ... but still looking for one |
| Lisa | should be back |
| Caroline | microscope |
| Souf | |
| Vicard | Lab |
| Pierre | microscope / Modeling |
| Sylvain | Run to Necker Hospital and come back with the primers. PCR |
| Guillaume | lab |
