Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(Digestion Products)
(Digestion Products)
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|style="background:#FFFF00"| D1
|style="background:#FFFF00"| D1
| P3
| P3
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P3]]BBa-J32015
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P3 P3]]BBa-J32015
| XbaI
| XbaI
| PstI
| PstI
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|style="background:#FFFF00"| D3
|style="background:#FFFF00"| D3
| P5
| P5
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P5]]BBa-I14032
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P5 P5]]BBa-I14032
| XbaI
| XbaI
| PstI
| PstI
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|style="background:#FFFF00"| D4
|style="background:#FFFF00"| D4
| P7
| P7
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P7]]Bba-R0040
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P7 P7]]Bba-R0040
| XbaI
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| PstI
| PstI
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|style="background:#FFFF00"| D5
|style="background:#FFFF00"| D5
| P8
| P8
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P8]]BBa-I0500
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P8 P8]]BBa-I0500
| XbaI
| XbaI
| PstI
| PstI
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|style="background:#FFFF00"| D6
|style="background:#FFFF00"| D6
| P2
| P2
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P2]] BBa-J61002
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P2 P2]] BBa-J61002
| XbaI
| XbaI
| PstI
| PstI

Revision as of 09:32, 12 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size Yes Yes 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size Yes Yes 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size Yes Yes 08/07
D10 ptet  ??? P18 XbaI PstI Size Yes Yes 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes Yes 08/10
D13 OmpAsignal  ??? A11 XbaI PstI ~135 Yes Yes 11/07
D14 TolRII  ??? A13 XbaI PstI ~279 Yes Yes 11/07