Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(New page: {{Template:Paris2009}} {{Template:Paris2009_menu}} ==Freezer== <center> Primers - PCR products - [[Team:Paris/Fr...)
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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Digestion_Products#bottom | Digestion Products]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
 
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<center>
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== Digestion Products ==
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[[Team:Paris/Freezer_Primers | Primers]] -
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<html>
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[[Team:Paris/Freezer_PCR_products | PCR products]] - 
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<style type="text/css">
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[[Team:Paris/Freezer_Plasmids | Plasmids]] -
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#left-side {
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[[Team:Paris/Freezer_Digestion_Products | Digestion Products]] -
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    position: absolute;
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[[Team:Paris/Freezer_Unexpected_digestion_results | Unexpected digestion results]] -
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    height: 23px;
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[[Team:Paris/Freezer_Strains_(Glycerol stock) | Strains (Glycerol stock)]]
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    width: 30px;
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    top: 0px;
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    left: 10px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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#middle-side {
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    height: 25px;
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    width: 660px;
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    position: absolute;
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    top: 0px;
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    left: 30px;
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    margin-top:10px;
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    padding-top: 5px;
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    background: #dadada;
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    z-index:5;
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}
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#right-side {
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    position: absolute;
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    height: 23px;
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    width: 30px;
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    margin-top:10px;
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    padding-top: 7px;
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    top: 0px;
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    left: 670px;
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    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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a.menu_sub {
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    padding-left: 7px;
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    padding-right: 7px;
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}
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a.menu_sub_active {
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    padding-left: 7px;
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    padding-right: 7px;
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    color:#b0310e;
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    font-weight:bold;
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}
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</style>
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<div id="left-side"></div>
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<div id="middle-side"><center>
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
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<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
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<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
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<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
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</div>
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<div id="right-side"></div>
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</html>
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<br>
<br>
{|
{|
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|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
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<html>
 
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</div>
 
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<div id="paris_content_boxtop">
 
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</div>
 
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<div id="paris_content">
 
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</html>
 
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== Digestion Products ==
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<div style="background-color:#FF0000; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Red = Vector</div>
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<div style="background-color:#FFFF00; padding:1px; margin:1px; font-size:120%; text-align:center; font-weight:bold; border:1px solid black">Yellow = Insert</div>
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{|
{|
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|- style="background: #0d3e99; text-align: center; color:white;"
|- style="background: #0d3e99; text-align: center; color:white;"
|width=5%| Number
|width=5%| Number
-
|width=20%| Product Name
+
|width=20%| Name
-
|width=20%| Matrix Name
+
|width=20%| Description
 +
|width=20%| Matrix ID
|width=5%| Enz. 1
|width=5%| Enz. 1
|width=5%| Enz. 2
|width=5%| Enz. 2
-
|width=5%| Size
+
|width=5%| Size (bp)
-
|width=20%| Description
+
|width=10%| Digested?
-
|width=10%| Product Left
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|width=10%| Purified?
-
|width=5%| []ng/µL
+
|width=5%| [ng/µL]
-
|width=5%| Date
+
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D1
|style="background:#FFFF00"| D1
| P3
| P3
-
| BBa-J32015
+
| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P3 P3]]BBa-J32015
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 66pb
-
| ???
+
| PelB leader sequence
-
| ???
+
| 47
| ???
| ???
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
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|style="background:#FFFF00"| D2
|style="background:#FFFF00"| D2
| P4
| P4
-
| BBa-B0014
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P4 P4]]BBa-B0014
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 95pb
-
| ???
+
| Double terminator bidirectionnal (B0012-B0011)
-
| ???
+
| 47
| ???
| ???
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
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|style="background:#FFFF00"| D3
|style="background:#FFFF00"| D3
| P5
| P5
-
| BBa-I14032
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P5 P5]]BBa-I14032
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 37pb
-
| ???
+
| pLac
-
| ???
+
| 47
| ???
| ???
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
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|style="background:#FFFF00"| D4
|style="background:#FFFF00"| D4
| P7
| P7
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| Bba-R0040
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P7 P7]]Bba-R0040
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 54pb
-
| ???
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| TetR repressible promote
-
| ???
+
| 47
| ???
| ???
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
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|style="background:#FFFF00"| D5
|style="background:#FFFF00"| D5
| P8
| P8
-
| BBa-10500
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P8 P8]]BBa-I0500
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 1210pb
-
| ???
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| pBad/araC
-
| ???
+
| 47
| ???
| ???
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D6
|style="background:#FFFF00"| D6
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| P2
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| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P2 P2]] BBa-J61002
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| XbaI
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| PstI
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| 2053bp (and 895bp)
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| Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet).
 +
| 47
| ???
| ???
 +
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D7
 +
| pSB2K3
| ???
| ???
 +
| P15
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D8
 +
| pSB1A3
| ???
| ???
 +
| P16
 +
| XbaI
 +
| PstI
 +
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D9
 +
| plbb RFP
| ???
| ???
 +
| P17
 +
| EcoRI
 +
| SpeI
 +
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D10
 +
| ptet
| ???
| ???
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
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| P18
 +
| XbaI
 +
| PstI
 +
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|style="background:#FFFF00"| D7
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|style="background:#FFFF00"| D11 <span/ id="D11">
-
| ???
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| RBS-tet
| ???
| ???
 +
| P19
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D12
 +
| pLac
 +
| In order to open the vector to add RBS-tet (XbaI/PstI)
 +
| P21
 +
| SpeI
 +
| PstI
 +
| Size
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/10_August_2009 08/10]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D13
 +
| OmpAsignal
| ???
| ???
 +
| A11
 +
| XbaI
 +
| PstI
 +
| ~135
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 11/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D14
 +
| TolRII
| ???
| ???
 +
| A13
 +
| XbaI
 +
| PstI
 +
| ~279
 +
| Yes
 +
| Yes
 +
| [https://2009.igem.org/Team:Paris/7_August_2009 11/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D15
 +
| double terminator
 +
| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P4 P4]]BBa-B0014
 +
| P4
 +
| EcoRI
 +
| SpeI
 +
| 95pb
 +
| Yes
| ???
| ???
-
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
+
| [https://2009.igem.org/Team:Paris/11_August_2009 11/08]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D16
 +
| pLac
 +
| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P21 P21]]BBa-I1014
 +
| P21
 +
| EcoRI
 +
| XbaI
 +
| ???
 +
| Yes
 +
| ???
 +
| [https://2009.igem.org/Team:Paris/11_August_2009 11/08]
|}
|}

Latest revision as of 15:26, 19 October 2009

iGEM > Paris > Freezer > Digestion Products


Digestion Products


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size Yes Yes 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size Yes Yes 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size Yes Yes 08/07
D10 ptet  ??? P18 XbaI PstI Size Yes Yes 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes Yes 08/10
D13 OmpAsignal  ??? A11 XbaI PstI ~135 Yes Yes 11/07
D14 TolRII  ??? A13 XbaI PstI ~279 Yes Yes 11/07
D15 double terminator [P4]BBa-B0014 P4 EcoRI SpeI 95pb Yes  ??? 11/08
D16 pLac [P21]BBa-I1014 P21 EcoRI XbaI  ??? Yes  ??? 11/08

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Digestion_Products"