Team:Paris/Freezer Plasmids

From 2009.igem.org

(Difference between revisions)
(New page: {{Template:Paris2009}} {{Template:Paris2009_menu}} ==Freezer== <center> Primers - PCR products - [[Team:Paris/Fr...)
 
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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Plasmids#bottom | Plasmids]]
{{Template:Paris2009}}
{{Template:Paris2009}}
{{Template:Paris2009_menu}}
{{Template:Paris2009_menu}}
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==Freezer==
 
-
<center>
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== Plasmids==
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[[Team:Paris/Freezer_Primers | Primers]] -
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<html>
-
[[Team:Paris/Freezer_PCR_products | PCR products]] - 
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<style type="text/css">
-
[[Team:Paris/Freezer_Plasmids | Plasmids]] -
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#left-side {
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[[Team:Paris/Freezer_Digestion_Products | Digestion Products]] -
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    position: absolute;
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[[Team:Paris/Freezer_Unexpected_digestion_results | Unexpected digestion results]] -
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    height: 23px;
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[[Team:Paris/Freezer_Strains_(Glycerol stock) | Strains (Glycerol stock)]]
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    width: 30px;
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    top: 0px;
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    left: 10px;
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    margin-top:10px;
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    padding-top: 7px;
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    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
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    z-index:4;
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}
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#middle-side {
 +
    height: 25px;
 +
    width: 650px;
 +
    position: absolute;
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    top: 0px;
 +
    left: 30px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
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    z-index:5;
 +
}
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#right-side {
 +
    position: absolute;
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    height: 23px;
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    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
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    top: 0px;
 +
    left: 670px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
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    z-index:4;
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}
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a.menu_sub {
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    padding-left: 7px;
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    padding-right: 7px;
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}
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a.menu_sub_active {
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    padding-left: 7px;
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    padding-right: 7px;
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    color:#b0310e;
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    font-weight:bold;
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}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
</center>
</center>
 +
</div>
 +
 +
<div id="right-side"></div>
 +
 +
</html>
 +
<br>
<br>
{|
{|
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|width=10% style="background: #FF0066;"| Chloranphenicol
|width=10% style="background: #FF0066;"| Chloranphenicol
|}
|}
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<html>
 
-
</div>
 
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<div id="paris_content_boxtop">
 
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</div>
 
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<div id="paris_content">
 
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</html>
 
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== Plasmids: MiniPrep Products ==
+
 
{|
{|
|- style="background: #c5c5c5;"
|- style="background: #c5c5c5;"
Line 44: Line 96:
|width=5%| Date
|width=5%| Date
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P1
+
|width=5%| P1 <span/ id="P1">
-
|width=25%| pSB2K3
+
|width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
|width=50%| General plasmid for cloning step after PCR
|width=50%| General plasmid for cloning step after PCR
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P2
+
|width=5%| P2 <span/ id="P2">
-
|width=25%| Bba-J61002
+
|width=25%| [http://partsregistry.org/Part:BBa_J61002 Bba_J61002]
|width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter
|width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P3
+
|width=5%| P3 <span/ id="P3">
-
|width=25%| Bba-J32015
+
|width=25%| [http://partsregistry.org/Part:BBa_J32015 Bba_J32015]
|width=50%| PelB leader sequence
|width=50%| PelB leader sequence
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P4
+
|width=5%| P4 <span/ id="P4">
-
|width=25%| Bba-B0014
+
|width=25%| [http://partsregistry.org/Part:BBa_B0014 Bba_B0014]
|width=50%| Double terminator bidirectionnal (B0012-B0011)
|width=50%| Double terminator bidirectionnal (B0012-B0011)
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P5
+
|width=5%| P5 <span/ id="P5">
-
|width=25%| Bba-I14032
+
|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032]
|width=50%| pLac
|width=50%| pLac
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P6
+
|width=5%| P6 <span/ id="P6">
-
|width=25%| BBa_K103006
+
|width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006]
|width=50%| OmpA linker (464bp)
|width=50%| OmpA linker (464bp)
-
|width=15%| Products in use
+
|width=15%| Disposed
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P7
+
|width=5%| P7 <span/ id="P7">
-
|width=25%| Bba-R0040
+
|width=25%| [http://partsregistry.org/Part:BBa_R0040 Bba_R0040]
|width=50%| TetR repressible promoter
|width=50%| TetR repressible promoter
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P8
+
|width=5%| P8 <span/ id="P8">
-
|width=25%| Bba-I0500
+
|width=25%| [http://partsregistry.org/Part:BBa_I0500 Bba_I0500]
|width=50%| pBad/araC
|width=50%| pBad/araC
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P9
+
|width=5%| P9 <span/ id="P9">
|width=25%| &Delta;N-Syn1A
|width=25%| &Delta;N-Syn1A
|width=50%| ???
|width=50%| ???
Line 98: Line 150:
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P10
+
|width=5%| P10 <span/ id="P10">
|width=25%| His6-SNAP25
|width=25%| His6-SNAP25
|width=50%| ???
|width=50%| ???
Line 104: Line 156:
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P11
+
|width=5%| P11 <span/ id="P11">
|width=25%| VAMP2-HiB6
|width=25%| VAMP2-HiB6
|width=50%| ???
|width=50%| ???
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|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
-
|width=5%| P12
+
|width=5%| P12 <span/ id="P12">
|width=25%| g3p
|width=25%| g3p
|width=50%| g3p in pHEN2 plasmid
|width=50%| g3p in pHEN2 plasmid
|width=15%| Products in use
|width=15%| Products in use
|width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
|width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P13 <span/ id="P13">
 +
|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|width=50%| High copy BioBrick assembly plasmid
 +
|width=15%| Products in use
 +
|width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P14 <span/ id="P14">
 +
|width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050]
 +
|width=50%| RBS-tetR
 +
|width=15%| Products in use
 +
|width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P15 <span/ id="P15">
 +
|width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 +
|width=50%| plasmid backbone
 +
|width=15%| All have been digested and purified
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P16 <span/ id="P16">
 +
|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|width=50%| plasmid backbone
 +
|width=15%| All have been digested and purified
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P17 <span/ id="P17">
 +
|width=25%| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
 +
|width=50%| plasmid backbone with RFP
 +
|width=15%| All have been digested and purified
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P18 <span/ id="P18">
 +
|width=25%| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
 +
|width=50%| ptet
 +
|width=15%| All have been digested and purified
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P19 <span/ id="P19">
 +
|width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050]
 +
|width=50%| RBS-tet
 +
|width=15%| All have been digested and purified
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P20 <span/ id="P20">
 +
|width=25%| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
 +
|width=50%| RBS-lacI
 +
|width=15%| ...
 +
|width=5%| [https://2009.igem.org/Team:Paris/8_August_2009 08/08]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P21 <span/ id="P21">
 +
|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032]
 +
|width=50%| pLac
 +
|width=15%| Products in use
 +
|width=5%| [https://2009.igem.org/Team:Paris/10_August_2009 08/10]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P22 <span/ id="P22">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P23 <span/ id="P23">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P24 <span/ id="P24">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P25 <span/ id="P25">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P26 <span/ id="P26">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P27 <span/ id="P27">
 +
|width=25%|
 +
|width=50%|
 +
|width=15%|
 +
|width=5%|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| P28 <span/ id="P28">
 +
|width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006]
 +
|width=50%| OmpA linker (464bp)
 +
|width=15%| PCR @57°C+ 15s@72°C
 +
|width=5%| [https://2009.igem.org/Team:Paris/14_August_2009 08/14]
|}
|}

Latest revision as of 15:27, 19 October 2009

iGEM > Paris > Freezer > Plasmids


Plasmids


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol


Plasmids: MiniPrep Products
Number Name Description Products in use Date
P1 [http://partsregistry.org/Part:pSB2K3 pSB2K3] General plasmid for cloning step after PCR Products in use 08/03
P2 [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] Plasmid backbone, it allows us to change promoter RFP as promoter Products in use 08/03
P3 [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] PelB leader sequence Products in use 08/03
P4 [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] Double terminator bidirectionnal (B0012-B0011) Products in use 08/03
P5 [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] pLac Products in use 08/03
P6 [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] OmpA linker (464bp) Disposed 08/03
P7 [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] TetR repressible promoter Products in use 08/03
P8 [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] pBad/araC Products in use 08/03
P9 ΔN-Syn1A  ??? Products in use 08/03
P10 His6-SNAP25  ??? Products in use 08/03
P11 VAMP2-HiB6  ??? Products in use 08/03
P12 g3p g3p in pHEN2 plasmid Products in use 08/04
P13 [http://partsregistry.org/Part:pSB1A3 pSB1A3] High copy BioBrick assembly plasmid Products in use 08/06
P14 [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] RBS-tetR Products in use 08/06
P15 [http://partsregistry.org/Part:pSB2K3 pSB2K3] plasmid backbone All have been digested and purified 08/07
P16 [http://partsregistry.org/Part:pSB1A3 pSB1A3] plasmid backbone All have been digested and purified 08/07
P17 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] plasmid backbone with RFP All have been digested and purified 08/07
P18 [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] ptet All have been digested and purified 08/07
P19 [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] RBS-tet All have been digested and purified 08/07
P20 [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS-lacI ... 08/08
P21 [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] pLac Products in use 08/10
P22
P23
P24
P25
P26
P27
P28 [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] OmpA linker (464bp) PCR @57°C+ 15s@72°C 08/14

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Plasmids"