Revision as of 08:58, 16 October 2009

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Protocol Map

As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:

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    <h3>As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:</h3>
 </blockquote>
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        <td colspan="6" rowspan="8" bgcolor="#5D5D5D"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_04.gif" alt="" name="Untitled_1_04" width="439" height="407" border="0" id="Untitled_1_04" /></td>
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        <td width="162" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_05.gif" alt="" name="Untitled_1_05" width="162" height="32" border="0" id="Untitled_1_05" /></a></td>
        <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_06.gif" alt="" name="Untitled_1_06" width="176" height="32" border="0" id="Untitled_1_06" /></a></td>
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          <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/plasmidprep.gif"  width="338" height="84" rel="lightbox[pro]" title="Pureyield Plasmid Preparation - A culture of bacterial cells are grown up. The plasmids are then extracted from the cells."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_08.gif" width="338" height="87" border="0" /></a></td>
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        <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#plasmidprep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_09.gif" alt="" name="Untitled_1_09" width="171" height="24" border="0" id="Untitled_1_09" /></a></td>
        <td width="167" rowspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_10.gif" alt="" name="Untitled_1_10" width="167" height="30" border="0" id="Untitled_1_10" /></a></td>
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        <td colspan="3" rowspan="2" bgcolor="#5D5D5D">
          <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/pcr.gif"  width="338" height="84" rel="lightbox[pro]" title="Polymerase Chain Reaction - DNA replication is instigated to increase DNA concentration. (Invitrogen Platinum® Pfx)"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_13.gif" width="338" height="84" border="0" /></td>
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        <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#pcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_17.gif" alt="" name="Untitled_1_17" width="171" height="35" border="0" id="Untitled_1_17" /></a></td>
        <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_18.gif" alt="" name="Untitled_1_18" width="167" height="35" border="0" id="Untitled_1_18" /></a></td>
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          <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/digest.gif"  width="338" height="84" rel="lightbox[pro]" title="Digestion - DNA strands are cut to either remove a sequence of base pairs or open up a plasmid."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_22.gif" width="338" height="150" border="0" /></td>
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        <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_23.gif" alt="" name="Untitled_1_23" width="143" height="51" border="0" id="Untitled_1_23" /></a></td>
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        <td colspan="2" rowspan="6" bgcolor="#5D5D5D">
          <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_24.gif" alt="" name="Untitled_1_24" width="329" height="324" border="0" id="Untitled_1_24" /></td>
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        <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#digestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_25.gif" alt="" name="Untitled_1_25" width="162" height="40" border="0" id="Untitled_1_25" /></a></td>
        <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_26.gif" alt="" name="Untitled_1_26" width="176" height="40" border="0" id="Untitled_1_26" /></a></td>
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          <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/ligate.gif"  width="338" height="88" rel="lightbox[pro]" title="Ligation - Two samples of DNA are joined together."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_28.gif" width="338" height="88" border="0" /></td>
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        <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_30.gif" alt="" name="Untitled_1_30" width="167" height="36" border="0" id="Untitled_1_30" /></a></td>
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Team:Southampton/Project/Protocols

From 2009.igem.org

Revision as of 08:58, 16 October 2009

Protocol Map

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