From 2009.igem.org

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Biobrick Parts: Rock Paper Scissors

For a list of all our parts, please click here.

To view the parts used in our Game of Life sub-project, click here.

Part Name	Type	Description	Team Favourite?	Length
K180000	Regulatory	Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.	Yes	1447
K180002	Composite	tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.	Yes	2330
K180010	Composite	Rock-paper-scissors – Rock primary plasmid Primary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180013.	Yes	3888
K180013	Composite	Rock-paper-scissors – Rock secondary plasmid [rhl pR, LacI generator] Secondary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180010.	Yes	1369
K180014	Composite	Rock-paper-scissors – Paper primary plasmid Primary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180016.	Yes	3952
K180016	Composite	Rock-paper-scissors – Paper secondary plasmid [lux pR, LacI generator] Secondary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180014.	Yes	1371
K180017	Composite	Rock-paper-scissors – Scissors primary plasmid Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180020.	Yes	3858
K180020	Composite	Rock-paper-scissors – Scissors secondary plasmid [las pR, LacI generator] Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180017.	Yes	1473

K180003	Composite	tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.		2173
K180005	Composite	Paper – Primary plasmid (part 1) [LuxR generator] Part of the paper primary plasmid; constitutively produces LuxR.		979
K180008	Coding	mCherry (rights owned by Clontech) mCherry fluorescent protein – a derivative of the DsRed fluorescent protein with a short maturation time but lower relative brightness.		708
K180009	Coding	mBanana (rights owned by Clontech) mBanana fluorescent protein – a derivative of the DsRed fluorescent protein with a longer maturation time and lower relative brightness than mCherry.		708
K180011	Composite	Rock – Primary plasmid (part 1) [RhlR generator] Part of the rock primary plasmid; constitutively produces RhlR.		927
K180012	Composite	Rock – Primary plasmid (part 2) [tac pR, mCherry and LasI generator] Part of the rock primary plasmid; generates mCherry and LasI under tac pR regulation.		2953
K180015	Composite	Paper – Primary plasmid (part 2) [tac pR, GFP and RhlI generator] Part of the paper primary plasmid; generates GFP and RhlI under tac pR regulation.		2965
K180018	Composite	Scissors – Primary plasmid (part 1) [LasR generator] Part of the scissors primary plasmid; constitutively produces LasR.		921
K180019	Composite	Scissors – Primary plasmid (part 2) [tac pR, mBanana and LuxI generator] Part of the scissors primary plasmid; generates mBanana and LuxI under tac pR regulation.		2929

Team:Southampton/Parts/RPS

From 2009.igem.org

Biobrick Parts: Rock Paper Scissors

For a list of all our parts, please click here.

To view the parts used in our Game of Life sub-project, click here.

Part Name

Type

Description

Team Favourite?

Length

Regulatory

Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

Yes

1447

Composite

tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Yes

2330

Composite

Rock-paper-scissors – Rock primary plasmid Primary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180013.

Yes

3888

Composite

Rock-paper-scissors – Rock secondary plasmid [rhl pR, LacI generator] Secondary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180010.

Yes

1369

Composite

Rock-paper-scissors – Paper primary plasmid Primary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180016.

Yes

3952

Composite

Rock-paper-scissors – Paper secondary plasmid [lux pR, LacI generator] Secondary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180014.

Yes

1371

Composite

Rock-paper-scissors – Scissors primary plasmid Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180020.

Yes

3858

Composite

Rock-paper-scissors – Scissors secondary plasmid [las pR, LacI generator] Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180017.

Yes

1473

Composite

tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

2173

Composite

Paper – Primary plasmid (part 1) [LuxR generator] Part of the paper primary plasmid; constitutively produces LuxR.

979

Coding

mCherry (rights owned by Clontech) mCherry fluorescent protein – a derivative of the DsRed fluorescent protein with a short maturation time but lower relative brightness.

708

Coding

mBanana (rights owned by Clontech) mBanana fluorescent protein – a derivative of the DsRed fluorescent protein with a longer maturation time and lower relative brightness than mCherry.

708

Composite

Rock – Primary plasmid (part 1) [RhlR generator] Part of the rock primary plasmid; constitutively produces RhlR.

927

Composite

Rock – Primary plasmid (part 2) [tac pR, mCherry and LasI generator] Part of the rock primary plasmid; generates mCherry and LasI under tac pR regulation.

2953

Composite

Paper – Primary plasmid (part 2) [tac pR, GFP and RhlI generator] Part of the paper primary plasmid; generates GFP and RhlI under tac pR regulation.

2965

Composite

Scissors – Primary plasmid (part 1) [LasR generator] Part of the scissors primary plasmid; constitutively produces LasR.

921

Composite

Scissors – Primary plasmid (part 2) [tac pR, mBanana and LuxI generator] Part of the scissors primary plasmid; generates mBanana and LuxI under tac pR regulation.

2929

| Top | University of Southampton iGEM 2009

Hybrid promoter (trp & lac regulated – tac pR)
A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

tac pR testing plasmid (GFP)
Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Rock-paper-scissors – Rock primary plasmid
Primary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180013.

Rock-paper-scissors – Rock secondary plasmid
[rhl pR, LacI generator]
Secondary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180010.

Rock-paper-scissors – Paper primary plasmid
Primary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180016.

Rock-paper-scissors – Paper secondary plasmid
[lux pR, LacI generator]
Secondary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180014.

Rock-paper-scissors – Scissors primary plasmid
Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180020.

Rock-paper-scissors – Scissors secondary plasmid
[las pR, LacI generator]
Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180017.

tac pR testing plasmid (GFP) – basic
Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

Paper – Primary plasmid (part 1)
[LuxR generator]
Part of the paper primary plasmid; constitutively produces LuxR.

mCherry (rights owned by Clontech)
mCherry fluorescent protein – a derivative of the DsRed fluorescent protein with a short maturation time but lower relative brightness.

mBanana (rights owned by Clontech)
mBanana fluorescent protein – a derivative of the DsRed fluorescent protein with a longer maturation time and lower relative brightness than mCherry.

Rock – Primary plasmid (part 1)
[RhlR generator]
Part of the rock primary plasmid; constitutively produces RhlR.

Rock – Primary plasmid (part 2)
[tac pR, mCherry and LasI generator]
Part of the rock primary plasmid; generates mCherry and LasI under tac pR regulation.

Paper – Primary plasmid (part 2)
[tac pR, GFP and RhlI generator]
Part of the paper primary plasmid; generates GFP and RhlI under tac pR regulation.

Scissors – Primary plasmid (part 1)
[LasR generator]
Part of the scissors primary plasmid; constitutively produces LasR.

Scissors – Primary plasmid (part 2)
[tac pR, mBanana and LuxI generator]
Part of the scissors primary plasmid; generates mBanana and LuxI under tac pR regulation.