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Biobrick Parts: Game of Life

For a list of all our parts, please click here.

To view the parts used in our Rock Paper Scissors sub-project, click here.

Part Name	Type	Description	Team Favourite?	Length
K180000	Regulatory	Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.	Yes	1447
K180001	Coding	GFP-LVA fluorescent protein GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.	Yes	754
K180002	Composite	tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.	Yes	2330
K180004	Composite	Game of Life – Primary Plasmid Primary plasmid for Game of Life. Must be used in conjunction with part K180007.	Yes	3138
K180007	Composite	Game of Life – Secondary Plasmid [tac pR, LuxI generator] Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.	Yes	2195

K180003	Composite	tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.		2173
K180005	Composite	Game of Life – Primary plasmid (part 1) [LuxR generator] Part of the Game of Life primary plasmid; constitutively produces LuxR.		979
K180006	Composite	Game of Life – Primary plasmid (part 2) [lux pR, GFP and LacI generator] Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.		2151

Team:Southampton/Parts/GoL

From 2009.igem.org

Biobrick Parts: Game of Life

For a list of all our parts, please click here.

To view the parts used in our Rock Paper Scissors sub-project, click here.

Part Name

Type

Description

Team Favourite?

Length

Regulatory

Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

Yes

1447

Coding

GFP-LVA fluorescent protein GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.

Yes

754

Composite

tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Yes

2330

Composite

Game of Life – Primary Plasmid Primary plasmid for Game of Life. Must be used in conjunction with part K180007.

Yes

3138

Composite

Game of Life – Secondary Plasmid [tac pR, LuxI generator] Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.

Yes

2195

Composite

tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

2173

Composite

Game of Life – Primary plasmid (part 1) [LuxR generator] Part of the Game of Life primary plasmid; constitutively produces LuxR.

979

Composite

Game of Life – Primary plasmid (part 2) [lux pR, GFP and LacI generator] Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.

2151

| Top | University of Southampton iGEM 2009

Hybrid promoter (trp & lac regulated – tac pR)
A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

GFP-LVA fluorescent protein
GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.

tac pR testing plasmid (GFP)
Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Game of Life – Primary Plasmid
Primary plasmid for Game of Life. Must be used in conjunction with part K180007.

Game of Life – Secondary Plasmid
[tac pR, LuxI generator]
Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.

tac pR testing plasmid (GFP) – basic
Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

Game of Life – Primary plasmid (part 1)
[LuxR generator]
Part of the Game of Life primary plasmid; constitutively produces LuxR.

Game of Life – Primary plasmid (part 2)
[lux pR, GFP and LacI generator]
Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.